Example sentences for: non-fat

How can you use “non-fat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.

  • They were transferred immediately to a 3% non-fat milk/PBS/0.

  • The membrane was blocked with 3% non-fat dry milk (Bio-Rad), 0.1% tween-20 in TBS (pH 7.4), then cut at molecular weight marker 45 kDa.

  • Separated proteins were electroblotted onto nylon membranes (Millipore Corp., Bedford, MA) which were then incubated for 2 h in a blocking solution containing 5% non-fat dry milk in phosphate buffered saline containing 0.02% Tween-20.

  • 5% SDS-PAGE, transferred onto PVDF membranes (Bio-rad, Richmond, CA), then blocked with 5% BSA and 5% non-fat milk.


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