Words similar to non-fat

Example sentences for: non-fat

How can you use “non-fat” in a sentence? Here are some example sentences to help you improve your vocabulary:

Following incubation in blocking buffer (10 mM Tris-HCl, pH 7.5, containing 10% non-fat dry milk, 100 mM NaCl, and 0.1% Tween 20), the membranes were probed with an affinity-purified rabbit polyclonal IgG directed against caspase-8 (Caspase-8 p20, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1:200 dilution in blocking buffer, overnight at 4°C.
The arrays were rinsed briefly in a 3% non-fat milk/PBS/0.
Electrophoresis was performed in SDS-polyacrylamide gels [ 35 ] using the indicated amount of total cell protein [ 36 ] . After electrophoresis, the proteins were transferred to nitrocellulose paper in buffer containing 25 mM Tris-HCl, 192 mM glycine, 20% v/v methanol, and 0.01% SDS, pH 8.5 [ 37 ] . Residual protein binding sites on the nitrocellulose were blocked by incubation for 30 minutes in TBST (25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Tween-20) containing 4% non-fat dry milk.
The next day, unbound antigen is removed and the coated wells are blocked with 300 μl of 2% non-fat milk in PBS (NFM-PBS) for one hour at room temperature.
The membrane was blocked with 5% non-fat milk in Tris-buffered saline containing 0.15% Tween-20.