Example sentences for: non-fat

How can you use “non-fat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Next, the nitrocellulose was incubated in TBST containing 2% non-fat dry milk containing primary antibody directed towards the indicated protein.

  • The membrane was blocked with 5% non-fat milk in Tris-buffered saline containing 0.15% Tween-20.

  • Electrophoresis was performed in SDS-polyacrylamide gels [ 35 ] using the indicated amount of total cell protein [ 36 ] . After electrophoresis, the proteins were transferred to nitrocellulose paper in buffer containing 25 mM Tris-HCl, 192 mM glycine, 20% v/v methanol, and 0.01% SDS, pH 8.5 [ 37 ] . Residual protein binding sites on the nitrocellulose were blocked by incubation for 30 minutes in TBST (25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Tween-20) containing 4% non-fat dry milk.

  • The primary antibody was incubated in the 5% non-fat milk with the proteins on the membrane overnight at 4°C, and the protein of interest was visualized by the enhanced chemiluminescent method (ECL).

  • For Syk and PLCγ2 detection, filters were blocked with 5% non-fat dry milk (BioRad).


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