Example sentences for: non-fat

How can you use “non-fat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The primary antibody was incubated in the 5% non-fat milk with the proteins on the membrane overnight at 4°C, and the protein of interest was visualized by the enhanced chemiluminescent method (ECL).

  • The membrane was blocked with 3% non-fat dry milk (Bio-Rad), 0.1% tween-20 in TBS (pH 7.4), then cut at molecular weight marker 45 kDa.

  • Next, the nitrocellulose was incubated in TBST containing 2% non-fat dry milk containing primary antibody directed towards the indicated protein.

  • The membrane was blocked with 5% non-fat milk in Tris-buffered saline containing 0.15% Tween-20.

  • For Syk and PLCγ2 detection, filters were blocked with 5% non-fat dry milk (BioRad).


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