Words similar to non-fat

Example sentences for: non-fat

How can you use “non-fat” in a sentence? Here are some example sentences to help you improve your vocabulary:

Electrophoresis was performed in SDS-polyacrylamide gels [ 35 ] using the indicated amount of total cell protein [ 36 ] . After electrophoresis, the proteins were transferred to nitrocellulose paper in buffer containing 25 mM Tris-HCl, 192 mM glycine, 20% v/v methanol, and 0.01% SDS, pH 8.5 [ 37 ] . Residual protein binding sites on the nitrocellulose were blocked by incubation for 30 minutes in TBST (25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Tween-20) containing 4% non-fat dry milk.
Next, the nitrocellulose was incubated in TBST containing 2% non-fat dry milk containing primary antibody directed towards the indicated protein.
The next day, unbound antigen is removed and the coated wells are blocked with 300 μl of 2% non-fat milk in PBS (NFM-PBS) for one hour at room temperature.
For Syk and PLCγ2 detection, filters were blocked with 5% non-fat dry milk (BioRad).
Separated proteins were electroblotted onto nylon membranes (Millipore Corp., Bedford, MA) which were then incubated for 2 h in a blocking solution containing 5% non-fat dry milk in phosphate buffered saline containing 0.02% Tween-20.