Words similar to non-fat

Example sentences for: non-fat

How can you use “non-fat” in a sentence? Here are some example sentences to help you improve your vocabulary:

Next, the nitrocellulose was incubated in TBST containing 2% non-fat dry milk containing primary antibody directed towards the indicated protein.
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
After centrifugation to reduce the volume to approximately 10 μl (approximately 20 min), a 3% non-fat milk blocking solution was added to each Cy5-labeled solution such that 25 μl milk was added for each array to be generated from the mix.
After fixation the cell-bearing coverslips were incubated in 20% non-fat powdered milk in PBS to block non-specific protein-protein interactions for 2 hours at room temperature.
Electrophoresis was performed in SDS-polyacrylamide gels [ 35 ] using the indicated amount of total cell protein [ 36 ] . After electrophoresis, the proteins were transferred to nitrocellulose paper in buffer containing 25 mM Tris-HCl, 192 mM glycine, 20% v/v methanol, and 0.01% SDS, pH 8.5 [ 37 ] . Residual protein binding sites on the nitrocellulose were blocked by incubation for 30 minutes in TBST (25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Tween-20) containing 4% non-fat dry milk.