Words similar to non-denaturing

Example sentences for: non-denaturing

How can you use “non-denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

The duplex or heteroduplex substrates were prepared by mixing the labeled top strand with 4 fold excess of the unlabeled bottom strand, heating at 80°C for three minutes, cooling to room temperature and purified by electrophoresis in a 20% non-denaturing polyacrylamide gel with TBE buffer.
Samples were run in 6 % non-denaturing polyacrylamide gels at 120-150 V in Tris-glycine buffer (50 mM Tris pH 8.5, 380 mM glycine, 2 mM EDTA) for 3 to 5 h at 4°C.
A standard protocol was used for denaturing Southern blot hybridization [ 29 ] , while for non-denaturing hybridization, DNA was transferred to membranes under non-denaturing conditions.
Using non-denaturing Southern blot hybridization on planarian genomic DNA, we established that the PEVE Ul sequences hybridized to the lighter fragment, whereas Us probes stained the less mobile fragment (Figure 1).
Briefly, 10 μl of each sample were electrophoresed at 4°C and under non-denaturing conditions using a 10% polyacrylamide gel containing 0.05% gelatin.