Words similar to non-denaturing
Example sentences for: non-denaturing
How can you use “non-denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:
Samples were run in 6 % non-denaturing polyacrylamide gels at 120-150 V in Tris-glycine buffer (50 mM Tris pH 8.5, 380 mM glycine, 2 mM EDTA) for 3 to 5 h at 4°C.
Proteins were electroeluted from the non-denaturing gel as describe here [ 24 ] . Gels were fixed with fixing solution (50% methanol, 10% acetic acid) for 15 minutes, followed by staining in Coomassie staining solution (20% acetic acid, 0.025% Coomassie blue G-250) for one hour, followed by destaining in destaining solution (5% methanol, 10% acetic acid) [ 42 ] .
The duplex or heteroduplex substrates were prepared by mixing the labeled top strand with 4 fold excess of the unlabeled bottom strand, heating at 80°C for three minutes, cooling to room temperature and purified by electrophoresis in a 20% non-denaturing polyacrylamide gel with TBE buffer.
Therefore, proteins from induced E. coli were also separated under non-denaturing conditions with or without addition of DTT.
Protein-DNA complexes were separated on 5% or 7% non-denaturing polyacrylamide gels run at 250 V in 0.5× TBE and visualized after the gels were dried and autoradiographed.