Example sentences for: non-denaturing

How can you use “non-denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The radiolabeled PCR products were digested with Alu I or Hpa II, denatured and electrophoresed for 10-16 h at 500 V on non-denaturing 6% acrylamide gels at room temperature [ 24 ] . For RFLP analysis, PCR products were digested with Mbo II and analyzed by electrophoresis on non-denaturing 6% acrylamide gels at 500 V for 3-4 hours.

  • A standard protocol was used for denaturing Southern blot hybridization [ 29 ] , while for non-denaturing hybridization, DNA was transferred to membranes under non-denaturing conditions.

  • Samples were run in 6 % non-denaturing polyacrylamide gels at 120-150 V in Tris-glycine buffer (50 mM Tris pH 8.5, 380 mM glycine, 2 mM EDTA) for 3 to 5 h at 4°C.

  • Complete processing was also observed when the E. coli proteins were separated in non-denaturing and non-reducing gels.

  • Therefore, proteins from induced E. coli were also separated under non-denaturing conditions with or without addition of DTT.


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