Words similar to non-denaturing

Example sentences for: non-denaturing

How can you use “non-denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

Complete processing was also observed when the E. coli proteins were separated in non-denaturing and non-reducing gels.
Samples were run in 6 % non-denaturing polyacrylamide gels at 120-150 V in Tris-glycine buffer (50 mM Tris pH 8.5, 380 mM glycine, 2 mM EDTA) for 3 to 5 h at 4°C.
Proteins were electroeluted from the non-denaturing gel as describe here [ 24 ] . Gels were fixed with fixing solution (50% methanol, 10% acetic acid) for 15 minutes, followed by staining in Coomassie staining solution (20% acetic acid, 0.025% Coomassie blue G-250) for one hour, followed by destaining in destaining solution (5% methanol, 10% acetic acid) [ 42 ] .
The duplex or heteroduplex substrates were prepared by mixing the labeled top strand with 4 fold excess of the unlabeled bottom strand, heating at 80°C for three minutes, cooling to room temperature and purified by electrophoresis in a 20% non-denaturing polyacrylamide gel with TBE buffer.
A standard protocol was used for denaturing Southern blot hybridization [ 29 ] , while for non-denaturing hybridization, DNA was transferred to membranes under non-denaturing conditions.