Words similar to non-affected

Example sentences for: non-affected

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Three exons (9br, 23a and 48a) are known to be subjected to alternative splicing and these isoforms were also detected at the protein level with isoform-specific antibodies [ 9 10 ] . Four additional alternative transcripts were described (ex29-, ex30-, ex29/30- and the N-isoform) but no further analysis at the protein level was reported [ 11 12 ] . During mutation analysis in the NF1 -gene we disclosed several additional splice variants in which specific exons were skipped and that were also present in fresh lymphocytes of non-affected persons, albeit typically at a low level [ 13 ] . Several other studies also report the existence of lowly expressed splice NF1 variants.
The data presented were obtained in three independent experiments with fibroblasts from two keloid prone individuals (labeled as keloid D and keloid T), as well as with fibroblasts from one non-affected individual.
The entire NF1 -coding region from two normal non-affected individuals was amplified in 5 overlapping segments by RT-PCR according to Heim [ 17 ] starting from fresh blood leukocytes.