Words similar to non-adherent
Example sentences for: non-adherent
How can you use “non-adherent” in a sentence? Here are some example sentences to help you improve your vocabulary:
In addition, we have constitutively overexpressed IDO in adherent and non-adherent cell lines in vitro.
After incubation, non-adherent cells were collected, washed in PBS, and CD4 and CD8 T-cell subsets were separated by high-affinity negative selection using CD4 and CD8 subset columns (R&D System, Minneapolis, MN) according to the manufacturer's instructions.
Adherent and non-adherent cells were dissolved in Tris-buffered saline (pH 7.6) containing 10 mM EDTA and 0.5% sarkosyl, and 1 mg/ml proteinase K. After digestion (~16 h) of cellular proteins at 55°C, DNA was sequentially extracted with phenol/chloroform and chloroform.
Adherent and non-adherent cells were washed three times with PBS, differential counts were carried out by automated counting (Cell Dyn 4000, Abbott Diagnostics, Santa Clara, CA) and cells were resuspended in lysis buffer for RNA isolation (RNeasy, Qiagen, Valencia, CA).
The phenotype induced by ADP-ribosylation of Rho in T lymphocytes appeared different than that induced in fibroblast models, where microinjection of C3 results in fibroblast retraction of pseudopodia, with the cell body rounding up eventually to become non-adherent [ 17 ] . Video time-lapse microscopy demonstrated the elongation of aberrant pseudopods in HPB-ALL cells plated on the β1 substrate VCAM-1, rather than an altered retraction of already formed pseudopods.