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Example sentences for: nodl

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Additional analyses of the putative nodL gene were conducted with Version 3.0b4 of the MrBayes software [ 56 ] . For these analyses, the Jones-Taylor-Thornton model of amino-acid replacement [ 57 ] was adopted and variation of replacement rates among sites was incorporated by a discretized gamma distribution with four rate categories [ 58 ] . Each Markov chain Monte Carlo analysis used four heated chains and employed a burn-in period of 10,000 cycles, followed by 990,000 additional cycles.
This value is strikingly different for the nodL genes in Rhizobium leguminosarum (57% G+C) and Mesorhizobium loti (68% G+C).
To provide experimental evidence that the Meloidogyne nodL sequences represent nematode loci, we cloned and sequenced a full-length transcript from M. incognita ( Mi-NodL ). Identification of the SL1 trans -splice leader at the 5' end of the message [ 41 ] , and a poly(A) tail at the 3' end, confirmed that this is a bona fide nematode gene (Figure 3).
The protein alignment of the M. incognita and R. leguminosarum nodL sequences was trimmed such that only identical amino acids remained, and the sequences back-translated, retaining the correct codon usage.
However, four of these genes do map to the same M. loti linear chromosome (Figure 4), including nodL and the glutamine synthetase gene, both of which are involved in nodulation/nitrogen fixation in rhizobia.