Words similar to nnr
Example sentences for: nnr
How can you use “nnr” in a sentence? Here are some example sentences to help you improve your vocabulary:
Thus, we predict that an even lower concentration of dsRNA, perhaps in the sub-nanomolar range, might be able to destroy NNR viral mRNAs that are rarer, such as those of RSV M2 and L proteins.
As described earlier (in the beginning of the Results section), intracellular NNR viral replication generates both negative- and positive-strand full-length genomic RNA, each of which should find complementarity to the appropriate strand of the anti-F dsRNA.
The problem is particularly acute for NNR viruses, which have large RNA genomes [ 8 9 ] . The 10-15 kb long RNA genomes of these viruses require specific sequences at the 5' and 3' termini for transcription and replication, and must be properly encapsidated by the nucleocapsid protein (N) in order to be recognized by the viral RdRP [ 15 16 ] . Thus, any recombinant technology must be able to faithfully reproduce these features of the genome.
These obstacles have been largely circumvented by the use of cloned viral cDNA that is then altered by standard DNA-based site-directed mutagenesis procedures [ 8 9 ] . Originally designed for influenza virus minigenomes [ 10 ] , such cDNA-based "reverse genetics" strategy has been adopted in a large number of NNR viruses, including vesicular stomatitis virus (VSV), respiratory syncytial virus (RSV), and measles, to name a few [ 11 12 13 14 ] . Recently, extension of this approach has resulted in the cloning of full-length viral cDNA capable of producing infectious recombinant virus particles upon transcription.
Using two different RSV gene mRNAs as targets, and another NNR virus (VSV) as well as cellular mRNAs as controls, we show that synthetic dsRNA molecules are highly efficient and specific silencers of cytoplasmic RNA viral gene expression.
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