Example sentences for: nick-translation

How can you use “nick-translation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • HPV or centromeric plasmid was labeled with biotin by nick-translation (Invitrogen Life Technologies, Carlsbad, CA).

  • BAC and PAC DNAs, isolated from bacterial cultures (Nucleobond, Clontech, Franklin Lakes, NJ), were labeled by nick-translation with biotin and digoxigenin.

  • This problem remained despite repeat preparation of plasmid by column extraction (Qiagen), repeat nick-translation of probe to yield different sized fragments, and repeat purifications of labeled probe through mini-columns (Qiagen) or by isopropanol precipitation.

  • Appropriate length (50 to 500 bp) of the nick translated fragments was monitored by checking the nick-translation products on a 1 % agarose gel.

  • Plasmid/insert DNA sequences were isolated from bacterial cultures by column purification (Qiagen, Valencia, CA) and were biotin-labeled by nick-translation (Invitrogen, Carlsbad, CA).


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