Words similar to nick-translation
Example sentences for: nick-translation
How can you use “nick-translation” in a sentence? Here are some example sentences to help you improve your vocabulary:
BAC and PAC DNAs, isolated from bacterial cultures (Nucleobond, Clontech, Franklin Lakes, NJ), were labeled by nick-translation with biotin and digoxigenin.
This problem remained despite repeat preparation of plasmid by column extraction (Qiagen), repeat nick-translation of probe to yield different sized fragments, and repeat purifications of labeled probe through mini-columns (Qiagen) or by isopropanol precipitation.
Appropriate length (50 to 500 bp) of the nick translated fragments was monitored by checking the nick-translation products on a 1 % agarose gel.
Plasmid/insert DNA sequences were isolated from bacterial cultures by column purification (Qiagen, Valencia, CA) and were biotin-labeled by nick-translation (Invitrogen, Carlsbad, CA).
The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).