Words similar to nfatp

Example sentences for: nfatp

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Most of the many phosphorylation sites in NFATp are in the N-terminal region of the protein [ 15, 27].
Sequence comparison of NFAT proteins revealed that the DNA-binding domain and a region referred to as the NFAT homology region (NHR) share sequence similarity, while other regions of the NFAT proteins share little or no sequence similarity (a schematic of NFATp is shown in Figure 1A) [ 1].
We chose to begin our analysis of NFAT proteins in vitro by working with human NFATp (B isoform, amino acids 1-921, Figure 1A).
Upon purifying recombinant HA-NFATp, we noticed that the protein migrated at an apparent molecular weight similar to the native NFATp in unstimulated T cells (approximately 140 kD when compared to size standards).
Recombinant HA-tagged NFATp proteins (approximately 500 ng) were incubated with calcineurin (500 ng, Sigma) and calmodulin (200 ng, Sigma) in 21 μl of buffer that had the following final concentrations of components: 7 mM Tris-HCl, 5 mM Hepes (pH 7.6), 90 mM NaCl, 15 mM betamercaptoethanol, 0.3 mM DTT, 1 mM EDTA, 30% (v/v) glycerol, 3 mM MgCl 2 , and 1.5 mM MnCl 2 . Control reactions were performed with NFATp proteins in the absence of added calcineurin and calmodulin.