Words similar to nfatp

Example sentences for: nfatp

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An analysis of the transcriptional properties unique to NFATp required the use of a reconstituted transcription system that is responsive to human NFATp, independent of AP-1 activity.
Recombinant HA-tagged NFATp proteins (approximately 500 ng) were incubated with calcineurin (500 ng, Sigma) and calmodulin (200 ng, Sigma) in 21 μl of buffer that had the following final concentrations of components: 7 mM Tris-HCl, 5 mM Hepes (pH 7.6), 90 mM NaCl, 15 mM betamercaptoethanol, 0.3 mM DTT, 1 mM EDTA, 30% (v/v) glycerol, 3 mM MgCl 2 , and 1.5 mM MnCl 2 . Control reactions were performed with NFATp proteins in the absence of added calcineurin and calmodulin.
We have also shown that recombinant NFATp purified from insect cells is a phosphoprotein that exhibits association with nuclei in vitro only after dephosphorylation by calcineurin.
First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
We therefore applied in vitro biochemical methods for the characterization of NFATp functional domains.