Words similar to neomycin
Example sentences for: neomycin
How can you use “neomycin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cortical astrocytes were harvested from neonatal C57BL/6 mice and infected with a replication-defective retrovirus encoding Tag and neomycin resistance as previously described [ 13 ] . The transferred viruses were SV40-6 [ 5 ] , in which wild type Tag is expressed from the M-MuLV LTR promoter; SV(X)T K1, which is constructed the same as SV40-6 except that the Tag is mutant [ 38 ] and defective for binding the retinoblastoma protein family [ 39 ] ; Linker-tkT, in which the cDNA for Tag is expressed from the herpes simplex 1 tk promoter [ 11 ] . The differentiation and transformation phenotypes of the cell lines utilizing the M-MuLV LTR promoter have been described [ 6 13 ] . The Linker-tkT lines (tkT) were either isolated as clones after G418 selection (as previously described) [ 6 ] or frozen immediately after selection (pooled clones) and clonally selected at a later time.
In addition to the GAL4-EGFP expression cassette, this plasmid contains the neomycin resistance gene under the control of the SV40 promoter and enhancer for selection of transfected cells in G418.
This approach is also appealing because it relies on reconstitution of active neomycin phosphotransferase activity, and so correctly targeted ES cell clones can be simply selected by growth in G418.
dNTP, deoxyribonucleoside triphosphate; hnRNP heterogeneous nuclear ribonuclear protein; nt, nucleotide; LTR, long terminal repeat; MEF, mouse embryonic fibroblast; NPT, neomycin phosphotransferase; RT PCR, reverse transcriptase polymerase chain reaction.
The 3' splice site is downstream of the initiation codon for neomycin phosphotransferase.
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