Example sentences for: neomycin

How can you use “neomycin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The mice used in this study were first described in 1995 [ 31 ] . Disruption of the first coding exon with a promterless β-galactosidase/neomycin resistance fusion cassette [ 33 ] created a null allele for Lifr . AB1 ES cells, on a129S7/SvEvBrd- Hprt b - m 2background, were used for targeting Lifr . The mice were sent to the Induced Mutant Resource of the Jackson Laboratory in 1994, from where one heterozygous ( Lifr +/-) male was received in 1997 to re-establish the colony at the University of Washington.

  • In contrast, we predicted that homologous recombination between the truncated Neo sequences in the targeting vector and Neo sequences within Hnf3αΔpgk-Neo ES cells would reconstitute expression of active neomycin phosphotransferase and so confer resistance to G418.

  • This Neo fragment lacked sequences encoding the last 49 amino acids of neomycin phosphotransferase.

  • A Xho1/HindIII (blunt) cassette containing the Tn5 neomycin phosphotransferase ( Neo )gene, that could confer resistance to G418 and whose expression in mammalian cells was directed by a phosphoglycerate kinase-1 ( pgk) promoter, was introduced into an XbaI (blunt) site between the Hnf3α genomic sequences [ 16, 25].

  • Expression of the Neo gene involved splicing of some hnRNP A2/B1 transcripts to a cryptic splice acceptor site located 28 nucleotides downstream of the neomycin phosphotransferase (NTP) initiation codon.


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