Words similar to neomycin
Example sentences for: neomycin
How can you use “neomycin” in a sentence? Here are some example sentences to help you improve your vocabulary:
In addition to the GAL4-EGFP expression cassette, this plasmid contains the neomycin resistance gene under the control of the SV40 promoter and enhancer for selection of transfected cells in G418.
The mice used in this study were first described in 1995 [ 31 ] . Disruption of the first coding exon with a promterless β-galactosidase/neomycin resistance fusion cassette [ 33 ] created a null allele for Lifr . AB1 ES cells, on a129S7/SvEvBrd- Hprt b - m 2background, were used for targeting Lifr . The mice were sent to the Induced Mutant Resource of the Jackson Laboratory in 1994, from where one heterozygous ( Lifr +/-) male was received in 1997 to re-establish the colony at the University of Washington.
This approach is also appealing because it relies on reconstitution of active neomycin phosphotransferase activity, and so correctly targeted ES cell clones can be simply selected by growth in G418.
Along these lines, when the aminogylcosides (kanamycin, hygromycin B, paromycin and neomycin) due to their ability to inhibit protein synthesis by affecting ribosomal fidelity, or puromycin because of its competing ability with tRNAs for binding on the large ribosomal subunit, or cycloheximide which inhibit the large ribosomal subunit by preventing ribosomal movement along the mRNA, were used in active HIV-1 infection, it was found that both cycloheximide and puromycin produced the greatest decrease in HIV-1 inhibition, presumably by inhibiting the large subunit of the ribosome [ 41 ] .
Adherent cells were transduced with an amphotropic retrovirus, DFG-hIL-1β-neo, which encodes the mature form of human IL-1β fused to the leader sequence of human parathyroid hormone to enable efficient secretion, and neomycin phosphotransferase [ 21 ] . Retroviral transductants were positively selected in complete DMEM containing Geneticin at 0.5 mg/ml.
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