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Example sentences for: nccit
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Samples from induced and control NCCIT cells were mixed and co-hybridized to array slides containing 22,648 different human cDNAs, representing 17,083 different Unigene clusters and including 7,230 characterized genes.
Moreover, we found that a luciferase reporter gene, placed under the control of the Follistatin promoter, was activated by Wnt-3A protein added to transiently transfected NCCIT cells (Figure 5).
As has been reported before for other cells [ 11 ] , MSX1, MSX2 and ID2 are elevated by BMP-4 in NCCIT cells (Figure 3).
To stimulate the NCCIT cells with Wnt, we used tissue culture medium containing active Wnt-3A protein produced by mouse L cells (Wnt-3A CM [ 8 ] ), in comparison to control conditioned medium (CCM).
The tissue culture supernatant was added to NCCIT cells [ 7 ] .