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Example sentences for: nawrath

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Pseudomonas syringae was cultured according to published methods [ 31 ] . Arabidopsis was grown in a HTPC walk-in chamber (Conviron, Winnipeg, MN) or a MTR-30 reach-in chamber (Conviron, Winnipeg, MN) as previously described [ 4 ] . Columbia, ndr1-1 and ndr1-1/gl-1 seed used were previously described [ 4 5 ] . Seeds of npr1-2 and sid2-1 mutants were generously provided by Dr. Jane Glazebrook (Torrey Mesa Research Institute) and Dr. Christiane Nawrath (University of Fribourg), respectively.
Samples were flash frozen in liquid nitrogen and total RNA was isolated according to Nawrath and Métraux [ 20 ] . RNA concentration was determined spectrophotometrically.