Words similar to napo

Example sentences for: napo

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For down-regulation experiments the cells were re-homogenized in 5 ml of 25 mM NaPO 4 buffer (pH 7.4).
5% Nonidet-P 40, 50 mM HEPES pH 7.4, 5 mM EDTA, 50 mM NaCl, 10 mM NaPO
113 nM) and similar to the previously reported K d of 0.056 nM for the wild-type alpha-2 receptor for [ 3H]rauwolscine in NaPO 4 buffer [ 23 ] . Inhibition experiments with [ 3H]rauwolscine indicated that the affinity of norepinephrine for the mutated receptors was approximately two-fold higher (lower K d value) than for wild-type receptor (Figure 3).
Briefly, filters were pre-hybridized at 65°C in hybridization buffer (5x SSC, 50 mM NaPO 4 , pH 7.4, 2.5x Denhardt's Solution) with 100 μg/ml denatured salmon sperm DNA for two hours.
For saturation experiments, the resulting pellet (a crude particulate membrane fraction) was resuspended in 200 volumes of 25 mM NaPO 4 buffer (pH 7.4) and re-homogenized for use in the radioligand binding assay.