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Example sentences for: namalwa

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Unlabeled DQA1*0101 oligonucleotides successfully competed for strong binding activity seen in nuclear extracts from the Namalwa B cell line (Fig.
5-μl PCR reaction, with 0.2 mM dNTP, 1.5 mM MgCl 2 , 5 μl 10X PCR Buffer (Invitrogen, Carlsbad, CA, USA), and 1 μg Namalwa genomic DNA.
Preincubation of Namalwa cell nuclear extracts with antibodies to NF-YA also retards the binding complex (Fig.
1) first led us to investigate whether DEK in nuclear extracts binds to MHC class II Y-box regulatory elements in vitro . With a double-stranded oligonucleotide probe containing the HLA-DQA1*0101 Y-box sequence, EMSAs revealed similar binding activity in nuclear extracts from B lymphoid (Namalwa), T lymphoid (Jurkat, CEM), monocytoid (U937), and HeLa cell lines (Fig.