Words similar to mycrab

Example sentences for: mycrab

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The localization of GR-GFP was determined in comparison to mycRab24, detected with anti-myc monoclonal antibody and rhodamine-conjugated GAM-IgG, as described above.
Cells were transfected with pCMV5 expression vectors encoding mycRab24(D123I) as described earlier.
Rab24 contains a unique arginine-rich insert, 126EEDRRRRR 133 [ 25 ] in the domain cognate to loop-8 in H-Ras [ 34 ] . The latter sequence is reminiscent of unusual arginine-rich nuclear localization signals that allow proteins like HIV tet and rev to undergo nuclear translocation by association with importin-β, in the absence of importin-α [ 45 46 ] . Since the loop-8 insert in Rab24 lies adjacent to the conserved N(T)KxD nucleotide binding cassette, we hypothesized that conformational changes caused by the D123I substitution might render the Rab24 mutant capable of disrupting the nuclear import machinery [ 47 ] . To test this possibility, we co-expressed mycRab24wt or mycRab24(D123I) with GR-GFP, a fusion between the glucocorticoid receptor and green fluorescent protein.
10B) showed substantial overlap with nuclear and cytoplasmic aggregates containing mycRab24(D123I).
However, when GR-GFP was coexpressed with mycRab24(D123I) there was a striking perturbation of receptor localization.