Words similar to mycrab

Example sentences for: mycrab

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The possibility that the inclusions induced by mycRab24(D123I) contain aggregated nuclear import complexes was further supported by immuno-staining with an antibody against importin-β (karyopherin-β), which revealed focal concentrations of importin-β in these structures (Fig.
To assess the guanine nucleotide state of selected mycRab24 proteins, cells were transfected with the indicated constructs and incubated with 32P-orthophosphate for 5 h, starting 24 h after transfection.
The localization of GR-GFP was determined in comparison to mycRab24, detected with anti-myc monoclonal antibody and rhodamine-conjugated GAM-IgG, as described above.
However, in some cells with larger and more numerous inclusions we observed discontinuities in the anti-lamin staining of the nuclear envelope and some co-localization of lamin with mycRab24(D123I) in the intranuclear aggregates.
Although Rab24 has been proposed as a possible regulator of autophagic processes [ 24 43 ] , the accumulation of punctate bodies in cells expressing Rab24(D123I) was not prevented by 3-methyadenine, a known inhibitor of autophagosome formation [ 44 ] . Most importantly, electron microscopy with immunogold labeling of mycRab24(D123I) in ultra-thin sections confirmed the initial impression that the inclusions are in fact localized within the nucleus.