Words similar to multicopy
Example sentences for: multicopy
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Approximately the same yield of gene-positive clones had been previously observed during TAR cloning of this genomic region with vectors lacking ARS and a counter-selectable marker [ 7 15 ] . No transgene-positive clones were found when 5-FOA selection was omitted (Table 3), indicating that the new TAR cloning method is highly selective and efficient for isolation of multicopy genes.
To further investigate the kinetics of heat shock and cadmium-induction of ApppN and AppppN levels, we transformed hnt2ΔADE2 strain BY71-6c with multicopy plasmids containing no HNT2 gene, the wild-type HNT2 gene, or the HNT2-His109Ala or HNT2-His109Asp alleles of HNT2 . Cultures were exposed to either 2 mM CdCl 2 or 46°C heat shock and intracellular concentrations of ApppN and AppppN were determined at 30-minute timepoints.
KRS1 on a multicopy plasmid showed no significant alteration of ApppN levels in any sample.
The functions of Boi1/Boi2 appear to be linked to those of Cdc42 and Rho3, which are Rho-type GTPases that are important for polarized cell growth [ 4 5 6 7 8 9 ] . Boi1 and Boi2 display two-hybrid interactions with both wild-type and mutationally activated Cdc42 but not with a mutant version of Cdc42 that is predicted to be impaired in the ability to bind nucleotide, suggesting that Boi1 and Boi2 may associate with the GTP-bound ("active") form of Cdc42 [ 1 ] . Further evidence that some function of Boi1 is linked to that of Cdc42 is that overexpression of Boi1 inhibits bud emergence and that this inhibition can be suppressed by overexpression of Cdc42 [ 1 2 ] . RHO3 can serve as a multicopy suppressor of the lethality caused by deletion of BOI1 and BOI2 [ 1 2 ] . These findings are consistent with the possibilities that Boi1 and Boi2 are targets of Cdc42 that promote cell growth in a manner that is regulated by Rho3.
However, deletion of lysU had no effect on heat-shock inducible AppppA accumulation [ 37 ] . The KRS1 gene [ 38 ] encoding cytosolic lysyl tRNA synthetase was cloned into multicopy plasmid pRS423 [ 39 ] to generate plasmid pM1.
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