Words similar to multicopy
Example sentences for: multicopy
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This reduction was active-site dependent: adding back multicopy HNT2 with the nucleophilic histidine replaced by alanine or aspartate reduced dinucleoside polyphosphate levels less than two-fold.
The functions of Boi1/Boi2 appear to be linked to those of Cdc42 and Rho3, which are Rho-type GTPases that are important for polarized cell growth [ 4 5 6 7 8 9 ] . Boi1 and Boi2 display two-hybrid interactions with both wild-type and mutationally activated Cdc42 but not with a mutant version of Cdc42 that is predicted to be impaired in the ability to bind nucleotide, suggesting that Boi1 and Boi2 may associate with the GTP-bound ("active") form of Cdc42 [ 1 ] . Further evidence that some function of Boi1 is linked to that of Cdc42 is that overexpression of Boi1 inhibits bud emergence and that this inhibition can be suppressed by overexpression of Cdc42 [ 1 2 ] . RHO3 can serve as a multicopy suppressor of the lethality caused by deletion of BOI1 and BOI2 [ 1 2 ] . These findings are consistent with the possibilities that Boi1 and Boi2 are targets of Cdc42 that promote cell growth in a manner that is regulated by Rho3.
As shown in Figure 2, tRNA-dependent lysine incorporation was increased 2.1-fold by expression of KRS1 from a multicopy plasmid.
Approximately the same yield of gene-positive clones had been previously observed during TAR cloning of this genomic region with vectors lacking ARS and a counter-selectable marker [ 7 15 ] . No transgene-positive clones were found when 5-FOA selection was omitted (Table 3), indicating that the new TAR cloning method is highly selective and efficient for isolation of multicopy genes.
Strain BY71-6c was transformed with plasmids pRS423 and pM1 (multicopy KRS1 ) and cultures were grown as for measurement of dinucleoside polyphosphate levels.
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