Example sentences for: mua
How can you use “mua” in a sentence? Here are some example sentences to help you improve your vocabulary:
We adopted a cDNA sequencing strategy that relies on an in vitro transposon insertion system based on the MuA transposase, combined with primer walking (see Materials and methods for details).
Transposon reactions consisted of 1 μl (50-150 ng) plasmid DNA isolated from Qiagen or Revprep DNA isolation robots, 1.6 μl 5× reaction buffer, 8 ng Entranceposon (Kan R), 0.4 μl MuA transposase, and deionized water to bring the final volume to 8 μl.
After heat inactivation of the MuA transposase, 2 μl of the reaction were used to transform 17 μl of DH5α chemically competent cells (Invitrogen) in 96-well format.
Loading...