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Example sentences for: msd
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Control charts of other variability and test performance measures, such as the MSD, standard deviation or CV of control responses, or average control response, also may be useful for reviewing tests and minimizing variability.
First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.
The net1(19m') but not net1(7m) allele showed a synthetic growth defect when combined with msd2-1 (Figure 6E).
, not all Cdc5 phosphorylation sites on Net1 were mapped and mutated), we evaluated whether the net1 phosphosite-mutants exhibited a synthetic growth phenotype when combined with msd2-1 (a mutant allele of CDC5 ). This experiment was based on the idea that a reduction in phosphorylation sites on Net1 coupled with a reduction in Cdc5 activity might synergize to prevent efficient release of Cdc14 during anaphase, resulting in failure to exit mitosis.
The equation and examples of MSD calculations are shown in Appendix C. PMSD may be calculated legitimately as a descriptive statistic for within-test variability, even when the hypothesis test is conducted using a non-parametric method.
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