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Example sentences for: mrnas

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The technique of RNAi, whereby the introduction of a sequence-specific double-stranded RNA leads to degradation of matching mRNAs [ 62 ] , has allowed the systematic surveying of thousands of C. elegans genes for phenotypes following transient gene knockout [ 63 64 65 ] . Such information is potentially transferable to understanding which genes have crucial roles in parasitic nematodes where high-throughput RNAi is not yet possible.
The gene-specific probes and primers for the quantitative detection of the target mRNAs were designed using Primer Express (PE Biosystems, Foster City, CA).
Purified mRNAs, [α - 32P]dATP, dNTP mix and reverse transcriptase were incubated at 50°C for 25 min for the synthesis of radioactively labeled cDNA probes.
These results imply that, in the synovium, regulation of IFN-γ and LT-α must vary in individual cells, even though both Th1 cytokine mRNAs can be expressed.
Stimulation of RAR α and γ mRNA in LNCaP cells by T differed from that in normal rat prostate and non-tumorigenic rat prostate epithelial cells (NRP-152), but was comparable to that in tumorigenic rat prostate epithelial cells (NRP-154) [ 13 14 ] . In cervical carcinoma cells, the RAR β gene was abnormally down-regulated by RA [ 36 ] . In ER positive human breast cancer cells, RAR α mRNA was also up-regulated by estrogen [ 16 ] . Thus, T stimulation of RAR mRNAs in LNCaP cells could be related to the pathologic state of the cells.