Words similar to mrlc
Example sentences for: mrlc
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4 for MRLC), blotted to PVDF or nitrocellulose membranes, immunostained with our rabbit polyclonal anti-HSP20 antibody (1:5000) or rabbit polyclonal anti-MRLC antibody (1:4000 in 1% bovine serum albumin and 0.01% sodium azide), and detected with enhanced chemifluorescence (ECF) for HSP20 and enhanced chemiluminescence (ECL) for MRLC [ 22 ] . A representative blot is shown in Fig.
5°C heat pretreatment of swine carotid artery increased ser 16-HSP20 phosphorylation and suppressed force, i.e., a reduction in force without a significant reduction in MRLC phosphorylation.
2) Deactivation mechanisms are the reversal of activation mechanisms: relaxation results from lower ser 19-MRLC phosphorylation induced by either reductions in [Ca 2+] [ 3 4 ] or increases in myosin light chain phosphatase activity [ 5 ] . Since dephosphoylated myosin does not cycle, force is reduced.
We found that temperatures between 43 and 46°C increased ser 16-HSP20 phosphorylation and suppressed force, i.e. reduced force without reductions in ser 19-MRLC phosphorylation (Fig.
The response can depend on the form of contractile activation, e.g. nitroglycerin reduces Ca 2+better after agonist activation compared with K +depolarization [ 20 ] . Comparison of ser 16-HSP20 phosphorylation/force relationships require careful analysis in comparison with ser 19-MRLC phosphorylation levels to clearly show that the change in force is force suppression rather than deactivation.