Words similar to mono
Example sentences for: mono
How can you use “mono” in a sentence? Here are some example sentences to help you improve your vocabulary:
The concentrated preparation was dialyzed against 50 mM Tris-HCl buffer (pH 8.4) containing 1 mM DTT overnight, loaded onto the same Mono Q column that had been equilibrated with the same Tris-HCl buffer.
When the major fractions with highest levels of activity were pooled and subjected to Mono Q chromatography as described above, two peaks of activity were again resolved, one in the wash fraction (minor) and one in the gradient fraction (major).
The protein fractions with enzymatic activity that eluted from the gradient of a hydroxylapatite column were chromatographed on a mono Q column and recovered as single peaks.
Identification of this activity as *AroQ, and not AroQ•PheA, was verified by elution of a single peak of chorismate mutase in the wash fraction recovered after Mono Q chromatography of the periplasmic fraction.
An FPLC-connected Mono Q HR 5/5 anion-exchange column (Pharmacia Biotech) equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT was loaded with the concentrated *AroQ fraction.