Words similar to mono
Example sentences for: mono
How can you use “mono” in a sentence? Here are some example sentences to help you improve your vocabulary:
When the major fractions with highest levels of activity were pooled and subjected to Mono Q chromatography as described above, two peaks of activity were again resolved, one in the wash fraction (minor) and one in the gradient fraction (major).
Identification of this activity as *AroQ, and not AroQ•PheA, was verified by elution of a single peak of chorismate mutase in the wash fraction recovered after Mono Q chromatography of the periplasmic fraction.
An FPLC-connected Mono Q HR 5/5 anion-exchange column (Pharmacia Biotech) equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT was loaded with the concentrated *AroQ fraction.
The protein fractions with enzymatic activity that eluted from the gradient of a hydroxylapatite column were chromatographed on a mono Q column and recovered as single peaks.
The concentrated preparation was dialyzed against 50 mM Tris-HCl buffer (pH 8.4) containing 1 mM DTT overnight, loaded onto the same Mono Q column that had been equilibrated with the same Tris-HCl buffer.