Words similar to monensin
Example sentences for: monensin
How can you use “monensin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Con A, phorbol myristate acetate (PMA), N-acetylcysteine (NAC), brefeldin A and monensin were obtained from Sigma (St.
5 ng/ml) as described previously [ 25 ] . Either monensin (1 uM) or brefeldin A (1 ug/ml) was used in all cases to block Golgi function and allow cytokine accumulation with the cell.
To each well, the following was added in order: 1 μl of 2 mM monensin (Sigma, St. Louis, Missouri, United States) in 100% EtOH, 100 μl of target cells, 100 μl of effector cells, and 1 μl of CD107-APC Abs.
These cells were treated with 10 μM Monensin and 10 μM Nigericine and equilibrated for 2 min with MES (4-morpholineethanesulfonic acid) calibration buffer with pHs from 4.0 to 7.0 prior to image acquisition.
The cytokine analysis relies on the intracellular accumulation of cytokines in the Golgi compartment over the 15 hr. activation period induced by either brefeldin A or monensin.