Words similar to monensin
Example sentences for: monensin
How can you use “monensin” in a sentence? Here are some example sentences to help you improve your vocabulary:
These cells were treated with 10 μM Monensin and 10 μM Nigericine and equilibrated for 2 min with MES (4-morpholineethanesulfonic acid) calibration buffer with pHs from 4.0 to 7.0 prior to image acquisition.
Con A, phorbol myristate acetate (PMA), N-acetylcysteine (NAC), brefeldin A and monensin were obtained from Sigma (St.
5 μM; Sigma), phytohemagglutinin (2 μg/ml; Wellcome Diagnostics, Greenville, NC, USA), and phorbol myristate acetate (20 ng/ml; Sigma) in the presence of monensin (2 μM; Sigma) for 6 h and were fixed in paraformaldehyde for subsequent intracellular cytokine analysis.
5 ng/ml) as described previously [ 25 ] . Either monensin (1 uM) or brefeldin A (1 ug/ml) was used in all cases to block Golgi function and allow cytokine accumulation with the cell.
The cytokine analysis relies on the intracellular accumulation of cytokines in the Golgi compartment over the 15 hr. activation period induced by either brefeldin A or monensin.