Words similar to mncl

Example sentences for: mncl

How can you use “mncl” in a sentence? Here are some example sentences to help you improve your vocabulary:

2 , 5 mM MnCl 2 , 100 μM sodium orthovanadate).
Phosphatase activities were assayed essentially as described [ 14 19 52 ] . Unless otherwise mentioned, 80 μl reactions contained 2 mM of MnCl 2 and requisite amount of recombinant enzyme in buffer A. Where mentioned, OA was directly added to the reaction.
Growth and induction of E. coli BL21(DE3) containing pET-15b-PfPP1 and the RIG plasmid were carried out using procedures described earlier [ 14 48 ] , except that the culture was grown at 18°C in the presence of 2 mM MnCl 2 , and IPTG concentration was lowered to 0.4 mM.
To examine the effect of divalent cation composition, 8 mM MnCl 2 , was used instead of 10 mM MgCl 2 , and again no RNAseH activity was detected (data not shown).
Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.