Example sentences for: mncl

How can you use “mncl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To examine the effect of divalent cation composition, 8 mM MnCl 2 , was used instead of 10 mM MgCl 2 , and again no RNAseH activity was detected (data not shown).

  • 2 , 5 mM MnCl 2 , 100 μM sodium orthovanadate).

  • Phosphatase activities were assayed essentially as described [ 14 19 52 ] . Unless otherwise mentioned, 80 μl reactions contained 2 mM of MnCl 2 and requisite amount of recombinant enzyme in buffer A. Where mentioned, OA was directly added to the reaction.

  • The (His) 6 -tagged PfPP1 expressed from pET-15b-PfPP1 was purified through Ni +2-chelation chromatography [ 14 ] as described by the manufacturer (Novagen), with 1 mM MnCl 2 being present in all the buffers.

  • Growth and induction of E. coli BL21(DE3) containing pET-15b-PfPP1 and the RIG plasmid were carried out using procedures described earlier [ 14 48 ] , except that the culture was grown at 18°C in the presence of 2 mM MnCl 2 , and IPTG concentration was lowered to 0.4 mM.


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