Words similar to mncl

Example sentences for: mncl

How can you use “mncl” in a sentence? Here are some example sentences to help you improve your vocabulary:

Growth and induction of E. coli BL21(DE3) containing pET-15b-PfPP1 and the RIG plasmid were carried out using procedures described earlier [ 14 48 ] , except that the culture was grown at 18°C in the presence of 2 mM MnCl 2 , and IPTG concentration was lowered to 0.4 mM.
The (His) 6 -tagged PfPP1 expressed from pET-15b-PfPP1 was purified through Ni +2-chelation chromatography [ 14 ] as described by the manufacturer (Novagen), with 1 mM MnCl 2 being present in all the buffers.
5, 0.5 % Triton X 100) and resuspended in Kinase Buffer - 20 mM Tris-HCl pH 7.5, 5 mM MgCl 2 and 5 mM MnCl 2 (all from Sigma-Aldrich, Inc.).
2 , 5 mM MnCl 2 , 100 μM sodium orthovanadate).
Phosphatase activities were assayed essentially as described [ 14 19 52 ] . Unless otherwise mentioned, 80 μl reactions contained 2 mM of MnCl 2 and requisite amount of recombinant enzyme in buffer A. Where mentioned, OA was directly added to the reaction.