Words similar to mncl

Example sentences for: mncl

How can you use “mncl” in a sentence? Here are some example sentences to help you improve your vocabulary:

5, 0.5 % Triton X 100) and resuspended in Kinase Buffer - 20 mM Tris-HCl pH 7.5, 5 mM MgCl 2 and 5 mM MnCl 2 (all from Sigma-Aldrich, Inc.).
The (His) 6 -tagged PfPP1 expressed from pET-15b-PfPP1 was purified through Ni +2-chelation chromatography [ 14 ] as described by the manufacturer (Novagen), with 1 mM MnCl 2 being present in all the buffers.
To examine the effect of divalent cation composition, 8 mM MnCl 2 , was used instead of 10 mM MgCl 2 , and again no RNAseH activity was detected (data not shown).
Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.
Phosphatase activities were assayed essentially as described [ 14 19 52 ] . Unless otherwise mentioned, 80 μl reactions contained 2 mM of MnCl 2 and requisite amount of recombinant enzyme in buffer A. Where mentioned, OA was directly added to the reaction.