Words similar to mmcop

Example sentences for: mmcop

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Compared to AtCOP1, the predicted full-length MmCOP1 and HsCOP1 proteins contain unique N-terminal glycine-serine rich extensions of about 70 amino acids (Fig.
pEGFP-N280 and pFLAG-N280 were generated by inserting a HindIII-StyI fragment (containing amino acid 70-350 of MmCOP1; StyI site was filled in by Klenow DNA polymerase) into pEGFP and pFLAG-CMV vectors digested by HindIII and SmaI.
We were also able to identify the full-length genomic sequence of mosquito COP1 (Anopheles gambiae COP1 , AgCOP1 ) and partial genomic sequences of MmCOP1 and fugu COP1 (Fugu rubripes COP1 , FrCOP1 ). The genomic structures of the vertebrate COP1 genes appear to be generally conserved among themselves, but different from mosquito and plant COP1 genes (data not shown).
Polyclonal antibodies against a N-terminal fragment of MmCOP1 (amino acid 71-270) were generated in rabbits and affinity purified.
The resulting construct was further digested with NotI and PstI and combined with a NotI-PstI fragment containing the remaining c-terminal portion of a previously described MmCOP1 cDNA clone (GenBank accession number AF151110.