Words similar to mmcop

Example sentences for: mmcop

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To obtain the full-length MmCOP1 cDNA, an EST clone (GenBank accession number AI509164) containing the N-terminal region of MmCOP1 cDNA in pT7T3D-Pac vector was obtained from ResGen and digested with XhoI and Hind III.
We were also able to identify the full-length genomic sequence of mosquito COP1 (Anopheles gambiae COP1 , AgCOP1 ) and partial genomic sequences of MmCOP1 and fugu COP1 (Fugu rubripes COP1 , FrCOP1 ). The genomic structures of the vertebrate COP1 genes appear to be generally conserved among themselves, but different from mosquito and plant COP1 genes (data not shown).
Notably, the corresponding site in AtCOP1 is within the mapped cytoplasmic localization sequence [ 17 ] . Moreover, the NLS must also be located within amino acids 71 to 270 of MmCOP1, since NES-mutated or LMB-treated N200 is nuclear localized (Fig 6B).
The resulting construct was further digested with NotI and PstI and combined with a NotI-PstI fragment containing the remaining c-terminal portion of a previously described MmCOP1 cDNA clone (GenBank accession number AF151110.
Comparison of the subcellular localization signals uncovers clear distinctions between AtCOP1 and MmCOP1.