Words similar to mmcop

Example sentences for: mmcop

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To obtain the full-length MmCOP1 cDNA, an EST clone (GenBank accession number AI509164) containing the N-terminal region of MmCOP1 cDNA in pT7T3D-Pac vector was obtained from ResGen and digested with XhoI and Hind III.
As another line of experiments, we generated expression constructs containing N-terminal GFP- and FLAG-tagged MmCOP1 and HsCOP1.
Interestingly in MmCOP1, the site matching the AtCOP1 NLS is found to be important for targeting the nuclear localized MmCOP1 deletion construct N346 to speckles rather than mediating nuclear import (Fig 8).
Furthermore, we also chose to mutate a forth positively charged amino acid cluster at amino acid 358 to 360, which corresponds to the Arabidopsis COP1 NLS [ 17 ] . Since GFP-N346 exhibited clear and consistent nuclear localized pattern with speckles most resembling the full-length MmCOP1 nuclear localization pattern, it was used as the starting point for the site-directed mutagenesis studies (Fig 7A).
Taken together, both the major nuclear and cytoplasmic localization signals seem to reside in the N-terminal region of MmCOP1, most likely between amino acid 71 and 350.