Words similar to mmcop
Example sentences for: mmcop
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However, GFP-ΔN70 does not display NE localization pattern, indicating that the first 70 amino acid of glycine-serine rich region may be responsible for targeting MmCOP1 to the NE (Fig 5Aand 5B).
To generate pEGFP-COP1, pKS-COP1 was digested with ApaI and XbaI and the resulting fragment containing the MmCOP1 cDNA was subcloned into the same sites of the pEGFP I vector.
To obtain the full-length MmCOP1 cDNA, an EST clone (GenBank accession number AI509164) containing the N-terminal region of MmCOP1 cDNA in pT7T3D-Pac vector was obtained from ResGen and digested with XhoI and Hind III.
1) encodes a protein product that corresponds to the full-length AtCOP1, but without the ATG start codon at its 5' end [ 20 ] . Homology search against the updated public EST database with the 5' end sequence of the available partial MmCOP1 cDNA allowed the identification of human and mouse EST clones containing a predicted starting codon and a partial 5' UTR, and the subsequent construction of full-length MmCOP1 and HsCOP1 cDNAs (see Experimental procedures for details).
Furthermore, we also chose to mutate a forth positively charged amino acid cluster at amino acid 358 to 360, which corresponds to the Arabidopsis COP1 NLS [ 17 ] . Since GFP-N346 exhibited clear and consistent nuclear localized pattern with speckles most resembling the full-length MmCOP1 nuclear localization pattern, it was used as the starting point for the site-directed mutagenesis studies (Fig 7A).