Example sentences for: mmcop

How can you use “mmcop” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Unlike GFP-ΔN70, GFP-ΔRING (amino acid 226-773) was only found in the cytoplasm in all the cells examined (Fig 5Aand 5B), suggesting that the NLS of MmCOP1 is probably located within amino acid 71 to 226 of MmCOP1.

  • Furthermore, we also chose to mutate a forth positively charged amino acid cluster at amino acid 358 to 360, which corresponds to the Arabidopsis COP1 NLS [ 17 ] . Since GFP-N346 exhibited clear and consistent nuclear localized pattern with speckles most resembling the full-length MmCOP1 nuclear localization pattern, it was used as the starting point for the site-directed mutagenesis studies (Fig 7A).

  • The resulting construct was further digested with NotI and PstI and combined with a NotI-PstI fragment containing the remaining c-terminal portion of a previously described MmCOP1 cDNA clone (GenBank accession number AF151110.

  • The previously reported partial MmCOP1 mRNA (GenBank accession number AF151110.

  • Taken together, both the major nuclear and cytoplasmic localization signals seem to reside in the N-terminal region of MmCOP1, most likely between amino acid 71 and 350.


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