Example sentences for: minus-strand

How can you use “minus-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • During synthesis of the minus-strand DNA the pregenomic RNA is degraded by the ribonuclease H (RNAseH) activity of P. However, the RNAseH activity does not digest the extreme 5' end of the template RNA, and this ∼ 18 nucleotide capped RNA fragment is then transferred to direct repeat 2 (DR2), where it acts as a primer for synthesis of plus-strand DNA [ 11, 12].

  • Transfection of cells with DNA carrying the D715V mutation inhibited DNA synthesis during minus-strand DNA synthesis, yielding immature minus-polarity DNAs (lane 3), and it also ablated positive polarity DNA synthesis (lane 7).

  • This nascent DNA then acts as a primer for the synthesis of full length minus-strand DNA templated by the pregenomic RNA [ 5, 6, 7, 8, 9, 10].

  • DNA synthesis arrests after 3-4 nucleotides, and then the protein-linked nascent minus-strand DNA is transferred to the copy of direct repeat 1 (DR1) within the 3' terminal duplication.


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