Words similar to millipore
Example sentences for: millipore
How can you use “millipore” in a sentence? Here are some example sentences to help you improve your vocabulary:
Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'
To remove excess unincorporated primers that would compete as sequencing primers in the cycle sequencing reaction, the amplified product is filtered with MultiScreen PCR filters (Millipore) and resuspended in 50 μL.
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
The samples from the coimmunoprecipitations described above were analyzed by electrophoresis on an SDS-12% polyacrylamide gel for 3 h at 160 V. The gel was equilibrated in transfer buffer (33 mM Tris base, 192 mM glycine, 20% methanol) for 15 min before transfer of proteins to Immobilon-P membrane (Millipore Corp.).
Samples were collected on 37 mm diameter 0.4-μm pore diameter polycarbonate membrane filters (Whatman Nucleopore Filtration Products, Clifton, New Jersey) in closed face polystyrene cassettes (Millipore, Bedford, Massachusetts) at 4 liters per minute (l/min) using sampling pumps in acoustic foam insulated boxes.
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