Words similar to millipore
Example sentences for: millipore
How can you use “millipore” in a sentence? Here are some example sentences to help you improve your vocabulary:
The electrophoresed samples were transferred onto ImmobilonP membranes (Millipore) by electroblotting for 1 hr in buffer containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, 10% methanol and 0.0025% Na dodecyl SO 4 . Prestained molecular mass markers (161-0372, BioRad) were supplemented with axonemal dynein from cilia of Tetrahymena (MM 528 kDa) in order to obtain a standard of sufficiently high molecular mass.
Deionized water may be obtained from MILLIPORE®, Milli-Q®, MILLIPORE QPAKTM2 or equivalent system.
Using these membranes, filtration binding assays were performed according to previously described protocols [ 28 ] . To reduce nonspecific binding, 96-well filtration plates (fiberglass FB filters; Millipore, Bedford, USA) were pre-coated as suggested by the manufacturer with 0.5% polyethyleneimine + 0.1% BSA and then washed four times with 0.1% BSA.
Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'
The uptake was stopped at 15 min. by the addition of 2 ml of ice-cold stop buffer (100 mM KCl, 100 mM mannitol, 10 mM potassium phosphate, pH 7.4) and the mitochondria were collected by rapid filtration on 0.45 μM Millipore membrane filters.
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