Example sentences for: millipore

How can you use “millipore” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The extracts, normalized for total protein content, were separated by SDS/PAGE and transferred to a polyvinylidene difluoride membrane (Immobilon-P, Millipore Corp.).

  • Proteins were transferred onto Immobilon P membrane (Millipore), blocked in 5 % nonfat dry milk dissolved in tris-buffered saline / 0.1 % Tween and probed with the various antibodies.

  • Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'

  • The fractions were concentrated by an Ultrafree-15 centrifugal filter device (Millipore Corporation), and loaded onto an FPLC-connected Superdex 75 HR 10/30 (Pharmacia Biotech) column which had been equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT and 150 mM NaCl.

  • The cDNA was purified using Millipore Microcon 30 centrifugal filter devices by diluting the reaction mix with sterile deionized water to a volume of 500 μl according to the manufacturers specifications.


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