Words similar to mii
Example sentences for: mii
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The origin of single, unpaired chromatids in MII oocytes has been proposed to result from premature separation of sister chromatids prior to anaphase I [ 56 57 ] . The possibility exists that both SC and aneuploidy may have originated from PCS of homologues and sister chromatids during the MAT of meiosis I. Upon removal of MG-132 from the culture media during meiosis I and the subsequent resumption of OM and proteasomal activity, faulty kinetochore-microtubule attachment accompanied by degradation of cohesion proteins could conceivably lead to PCS of homologues and sister chromatids.
0 mcg/ml MG-132 during meiosis I led to significant (P < 0.01) increases in the proportions of MI arrested oocytes and of MII oocytes with SC and hyperploidy.
PCS of homologous chromosomes and sister chromatids prior to establishing proper kinetochore-spindle attachment during meiosis I increases the probabilities of both homologues going to the same pole and of equational division of sister chromatids during anaphase I. Upon normal fertilization, MII oocytes with an additional chromosome result in trisomic zygotes; whereas oocytes containing an extra chromatid would be expected to produce 50% trisomic zygotes due to random segregation during anaphase II.
As described previously in this paper and elsewhere [ 38 ] , the biology of fission yeast facilitates the identification of MII segregation defects that are difficult to detect in other organisms that terminate meiotic progression or produce inviable meiotic products as a consequence of defects in meiotic chromosome dynamics.
Oocytes were collected and processed according to a mass-harvest procedure [ 63 ] and chromosomes were C-banded [ 64 ] to distinguish between complete dyads (MII chromosomes) and disjoined dyads (single chromatids).
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