Words similar to mid-log
Example sentences for: mid-log
How can you use “mid-log” in a sentence? Here are some example sentences to help you improve your vocabulary:
22 rad26-GFP strain was isolated and grown to mid-log phase before shifting to the restrictive temperature for 3 hours.
All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).
To test if Rad26-GFP localization changes in response to DNA damage, a culture of rad26-GFP cells was grown to mid-log phase (optical density 0.3) and then treated with Bleomycin, a radiomimetic drug, for 3 hours before fixation and DAPI staining.
Cells were grown to mid-log phase of growth (5.
ifkA null cells and Ax4 cells after starvation, mid-log phase cells growing axenically were collected, washed, and resuspended in the standard development buffer (PDF, 20 mM potassium phosphate, pH 6.1, 5 mM magnesium chloride, 0.5% streptomycin sulfate) at 5 × 10 6cells/ml.