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Example sentences for: microinjected
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For example, chronic treatment with fluoxetine decreases the release of AVP from rat hypothalamic organ cultures [ 36 ] . This finding is particularly noteworthy since alterations in AVP levels in the cerebrospinal fluid have been correlated with occurrences of OCD [ 15 16 ] . The occurrence of OCD has also been correlated with increased levels of oxytocin, a neuropeptide closely related to AVP [ 37 ] . In golden hamsters, the elevation of serotonin in the anterior hypothalamus following fluoxetine treatment can inhibit the activity of the AVP system related to aggressive behavior [ 10 11 ] . The ability of microinjected AVP in the anterior hypothalamus to enhance aggressive behavior in golden hamsters is blocked by fluoxetine treatment and serotonin 5HT 1A receptor agonists [ 10 ] . The present data include AVP-stimulated flank marking in the list of behaviors suppressed by fluoxetine treatment.
On the day of testing, animals were treated with clomipramine and 60-90 min later microinjected with 1.0 μM AVP in a volume of 100 nl and scored for flank marking and flank gland grooming.
A KpnI/XbaI digest liberated the CD2/hCARΔcyt transgene from the vector backbone and the transgene was subsequently microinjected into fertilized oocytes by the UAB ES/Transgenic facility.
To this end, dose-response data for clomipramine were generated in animals microinjected with 1.0 μM AVP.
Approximately 24 hours after antisense treatment, the cells were microinjected with pGR(Ala)-GFP and subjected to serum-starvation as described above.
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