Example sentences for: microcystin

How can you use “microcystin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Finally, it has been shown that affinity resins containing immobilized microcystin specifically bind toxin-sensitive phosphatases such as PP1 and PP2A [ 31 ] . Thus, we passed soluble cytosolic Pf extract through microcystin-Sepharose, and the bound proteins were analyzed on SDS-PAGE followed by immunoblot using the anti-PP1 antibody.

  • IVT proteins were incubated with the GST fusion proteins on beads for 1 hour at 4°C in 500 μL of 40 mM HEPES pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM EDTA, 1% Triton X100, 1 mM ATP, 1 μM microcystin, 14 mM b-mercaptoethanol.

  • Pre-incubation of the extract with microcystin specifically prevented binding of PfPP1 to the column.

  • The blot revealed that the 35 kDa PfPP1 polypeptide indeed specifically bound to microcystin (Fig.


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