Words similar to micrococcal
Example sentences for: micrococcal
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It has been demonstrated that malignant tissues with accelerated cell cycle show higher levels of ProTα expression than normal or surrounding healthy tissues [ 67 ] . ProTα was shown as a marker for breast cancer [ 68 ] , hepatocarcinoma [ 69 ] , and plasma levels of its derivative Tα 1 been proposed as a marker for the prognosis of lung cancer [ 70 ] . In ligand blotting assays, ProTα bound only to chromatin pools and nuclear fractions where histone H1 was present [ 71 72 ] . The analysis of the interaction of ProTα with H1-containing chromatin suggests a putative role for ProTα in the fine-tuning of the stoichiometry and/or mode of interaction of H1 with chromatin [ 73 ] . Interestingly, HL-60 cells overexpressing ProTα show an enhancement of accessibility of micrococcal nuclease to chromatin, implying relaxed chromatin structure for enhanced cell cycle gene expression [ 74 ] .
Control experiments demonstrated that (i) the cores were permeabilized because the viral nucleic acids were sensitive to micrococcal nuclease following low pH treatment; (ii) the RNAseH substrate could access the polymerase within the viral cores as determined by the trans reaction (Fig.
The cell lysate was clarified at 13,000×g for 5 min and digested with 100 units micrococcal nuclease (Roche) for 45 min at 37°C in the presence of 10 mM CaCl 2 . Digestion was stopped by addition of EDTA to 15 mM, and then the lysate was clarified again by centrifugation at 13,000×g for 5 min.
Nucleic acids within permeabilized cores were digested with micrococcal nuclease in the presence of CaCl 2 Micrococcal nuclease was inactivated by addition of EGTA, 1.5 μg RNA was added, and the reaction was performed under conditions described for the endogenous polymerase reaction.
∈ RNA was added in trans to micrococcal nuclease treated cores in the RNAseH assay, and again, DHBV RNAseH activity was not observed (data not shown).
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