Example sentences for: microcentrifuge

How can you use “microcentrifuge” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For preparation of protein extracts from rat, human and porcine tissues for western blot analysis, 150 cryostat tissue sections (7 μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.

  • Cell-free extracts were obtained by centrifugation in a microcentrifuge.

  • Extracts were briefly sonicated and then centrifuged at full speed in a microcentrifuge at 4°C for 15 min.

  • The lysate was then centrifuged at full speed in a microcentrifuge at 4°C for 15 min.

  • For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).


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