Words similar to microcentrifuge
Example sentences for: microcentrifuge
How can you use “microcentrifuge” in a sentence? Here are some example sentences to help you improve your vocabulary:
A total of 2,500 sorted cells (30 μl) and 250 target cells (30 μl) were transferred to a microcentrifuge tube (VWR International, West Chester, Pennsylvania, United States), centrifuged 1 min at 200 g, and incubated 4 h at 37 °C.
Cell lysates were transferred to fresh microcentrifuge tubes.
For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).
Cell extracts were spun at 14,000 rpm in a microcentrifuge and supernatants were incubated for 40-60 minutes with either anti-HA beads (Roche) or anti-v5 antibody (Invitrogen) and Protein A beads (Pierce; Rockford, USA) to form immunocomplexes.
For preparation of protein extracts from rat, human and porcine tissues for western blot analysis, 150 cryostat tissue sections (7 μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.
Loading...