Words similar to microcentrifuge
Example sentences for: microcentrifuge
How can you use “microcentrifuge” in a sentence? Here are some example sentences to help you improve your vocabulary:
After a 10 min spin at 14,000 rpm in a microcentrifuge, the supernatants were transferred to new tubes.
Cell lysates were transferred to fresh microcentrifuge tubes.
For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).
Cell extracts were spun at 14,000 rpm in a microcentrifuge and supernatants were incubated for 40-60 minutes with either anti-HA beads (Roche) or anti-v5 antibody (Invitrogen) and Protein A beads (Pierce; Rockford, USA) to form immunocomplexes.
The samples were then placed into microcentrifuge tubes containing 1 mL of sodium phosphate buffer (10 mmol/L, pH 7.4) and finely minced with scissors for 15 seconds.