Example sentences for: methylxanthine

How can you use “methylxanthine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The medium was replaced 24 hours later and incubated one additional hour at 37°C in 450 μL DMEM plus 0.25 mM 3-isobutyl-1-methylxanthine (IBMX), and 40 mM indomethacin.

  • The effector specificity could be rationally changed by changing the specificity of the conjoined aptamer: when a mutation was introduced into the anti-theophylline aptamer that led to discrimination against theophylline and in favor of 3-methylxanthine (that also differed from theophylline by a single methyl group), the aptamer became solely responsive to 3-methylxanthine in vivo . Group I aptazymes may have a number of important biotechnology applications, including use as in vivo , real-time reporters and as regulatable gene therapeutics.

  • [ 3H]-adenine-labeled cells were assayed for cAMP accumulation after incubation at 37°C for 40 min. in the presence of 1 mM 3-isobutyl-1-methylxanthine (IBMX) a phosphodiesterase inhibitor, and in the presence or absence of 7.5 ng/mL human chorionic gonadotropin (hCG), as described previously.

  • In contrast, the original aptamer was known to also bind 3-methylxanthine, and the original Group I aptazyme (Th2P6) also proved to be responsive to 3-methylxanthine in vivo (Figure 6c).

  • Stock solutions of forskolin (10 mM) and 3-isobutyl-1-methylxanthine (IBMX, 80 mM) were in DMSO.


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