Words similar to methylxanthine
Example sentences for: methylxanthine
How can you use “methylxanthine” in a sentence? Here are some example sentences to help you improve your vocabulary:
4-aminopyridine (4-AP); analysis of variance (ANOVA); 8-(4-chlorophenylthio)adenosine-3',5'-cyclic monophosphorothioate (8-CPT-cAMP); 4',4' diisothicyanato-stilbene-2-2'-disulfonic acid (DIDS); bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC 4 (3)); 5α-dihydrotestosterone (5α-DHT); Dulbecco's modified eagle's medium (DMEM); enhanced chemiluminescence (ECL); ethylenediaminetetraacetic acid (EDTA); fetal bovine serum (FBS); follicle stimulating hormone (FSH); granulosa cells (GC); human chorionic gonadotropin (hCG); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES); 3β-hydroxysteroid dehydrogenase (3β-HSD); 3-isobutyl-1-methylxanthine (IBMX); least significant difference (LSD); luteinizing hormone (LH); mitogen activated protein kinase (MAPK); 1,3-Benzenedicarboxylic acid, 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetrakis [(acetyloxy)methyl] ester (PBFI-AM); phosphate-buffered saline (PBS); proliferating cell nuclear antigen (PCNA); protein kinase A (PKA); sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE); steroidogenic acute regulatory protein (StAR); tris-buffered saline (TBS).
In contrast, the original aptamer was known to also bind 3-methylxanthine, and the original Group I aptazyme (Th2P6) also proved to be responsive to 3-methylxanthine in vivo (Figure 6c).
The medium was replaced 24 hours later and incubated one additional hour at 37°C in 450 μL DMEM plus 0.25 mM 3-isobutyl-1-methylxanthine (IBMX), and 40 mM indomethacin.
[ 3H]-adenine-labeled cells were assayed for cAMP accumulation after incubation at 37°C for 40 min. in the presence of 1 mM 3-isobutyl-1-methylxanthine (IBMX) a phosphodiesterase inhibitor, and in the presence or absence of 7.5 ng/mL human chorionic gonadotropin (hCG), as described previously.
The effector specificity could be rationally changed by changing the specificity of the conjoined aptamer: when a mutation was introduced into the anti-theophylline aptamer that led to discrimination against theophylline and in favor of 3-methylxanthine (that also differed from theophylline by a single methyl group), the aptamer became solely responsive to 3-methylxanthine in vivo . Group I aptazymes may have a number of important biotechnology applications, including use as in vivo , real-time reporters and as regulatable gene therapeutics.
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