Words similar to methylxanthine

Example sentences for: methylxanthine

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In contrast, the original aptamer was known to also bind 3-methylxanthine, and the original Group I aptazyme (Th2P6) also proved to be responsive to 3-methylxanthine in vivo (Figure 6c).
[ 3H]-adenine-labeled cells were assayed for cAMP accumulation after incubation at 37°C for 40 min. in the presence of 1 mM 3-isobutyl-1-methylxanthine (IBMX) a phosphodiesterase inhibitor, and in the presence or absence of 7.5 ng/mL human chorionic gonadotropin (hCG), as described previously.
To each well, phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) was added to a final concentration of 100 μM, followed by addition of agonists at different concentrations and incubation at 37°C.
In order to examine effector-specificity in vivo , a new series of Group I aptazymes was constructed in which the anti-theophylline aptamer was mutated to bind 3-methylxanthine (3MeX2P6) [ 34 ] . These variants proved to be responsive to 3-methylxanthine both in vitro and in vivo (Figure 6a).
The medium was replaced 24 hours later and incubated one additional hour at 37°C in 450 μL DMEM plus 0.25 mM 3-isobutyl-1-methylxanthine (IBMX), and 40 mM indomethacin.