Words similar to methionine-labeled

Example sentences for: methionine-labeled

How can you use “methionine-labeled” in a sentence? Here are some example sentences to help you improve your vocabulary:

After denaturation in 6 M and renaturation in 0.187 M guanidine-HCl in HB buffer (25 mM Hepes pH 7.2, 5 mM NaCl, 5 mM MgCl2, 1 mM DTT), the blots were saturated at 4°C in buffer H (20 mM Hepes pH 7.7, 7.75 mM KCl, 0.1 mM EDTA, 25 mM MgCl 2 , 1 mM DTT, 0.05% NP40, 1% dry milk) for 2 hours, and then incubated for 2 hours at 4°C with 50 μl of 35S-methionine-labeled in vitro-translated hCCR4 or luciferase protein (synthesized using a reticulocyte lysate-coupled transcription/translation kit [Promega]).
As a control, incubation with a 35S-methionine-labeled luciferase protein failed to show any interaction.
Bacterially produced, purified GST-CAF1 and control GST proteins were subjected to SDS-PAGE, transferred onto a membrane and probed with a 35S-methionine-labeled hCCR4 protein synthesized in vitro.