Words similar to mercaptoethanol

Example sentences for: mercaptoethanol

How can you use “mercaptoethanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

5 mM Tris-HCl, 2% SDS, 10% glycerol, 10% 2-mercaptoethanol, pH 6.8) at room temperature for 30 min in preparation for SDS-PAGE.
The reaction was performed in endogenous polymerase buffer (50 mM Tris-HCl pH 8.0, 100 mM NaCl, 0.1% NP-40, 10 mM MgCl 2 2.5% 2-mercaptoethanol) with 1 μg oligonucleotide, 100 ng purified radiolabeled RNA template, and 4 U RNasin at 37°C for 65 min.
The cultures were maintained in Minimum Essential Medium (MEM, GibcoBRL, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (Sigma, St. Louis, MO), 1.4 mM glutamine (GibcoBRL, Grand Island, NY) and 20 μM 2-mercaptoethanol (Sigma, St. Louis, MO).
The serine/threonine protein phosphatase type 5 (PP5) was purified from bovine brain according to the following procedure: a bovine brain was homogenized in ~ 3 volumes of buffer A (50 mM HEPES pH 7.5 4°C, 1 mM EDTA, 10 mM PMSF and 0.1% 2-mercaptoethanol) and subjected to centrifugation at 15,000 g for 40 min. followed by a 45%-60% ammonium sulfate fractionation of the supernatant.
125 M Tris, 2% SDS, 10% sucrose, 5% 2-mercaptoethanol, and 0.02% bromophenol blue at a pH of 8.0) was added to each tube and the tubes were heated at 95°C for 5 minutes.