Words similar to mercaptoethanol

Example sentences for: mercaptoethanol

How can you use “mercaptoethanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

1 mM 2-mercaptoethanol/1% penicillin-streptomycin) at 37°C/5% CO 2 until analysis.
Jurkat cells expressing SV40 large T antigen (Jurkat Tag) were a generous gift from Dr. Gerald Crabtree (Stanford) and were maintained in RPMI 1640 supplemented with 10% fetal calf serum, penicillin, streptomycin, glutamine, and 50 μM 2-mercaptoethanol.
Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.
Murine thymoma EL4 cells and murine CD5+ B cells, M12, were maintained in RPMI 1640 medium supplemented with 10% (v/v) 1:1 fetal calf serum and horse serum, 2 mM glutamine, 100 U/ml penicillin, 0.05 mM streptomycin, and 50 nM 2-mercaptoethanol.
32P-labeled substrates were prepared and assays performed as described previously [ 18 ] . Briefly, protein phosphatase reactions contained 2 or 4 nM enzyme in a final volume of 30 μl containing buffer A (50 mM Tris, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% 2-mercaptoethanol), 0.4% ethanol and 10 μM radiolabeled phosphosubstrate in the absence or presence of AA [ 19 ] . Reactions were initiated by adding substrate and lipid, and incubated at 30°C for 10 to 15 min.