Words similar to mercaptoethanol

Example sentences for: mercaptoethanol

How can you use “mercaptoethanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

For Western blot analysis, 15 μl of samples containing 20 μg protein were mixed with equal volume of 2 × loading buffer (1X = 125 mM TRIS-HCl, pH 6.8; 2% SDS, 5% glycerol, 0.003% bromphenol blue and 1% 1-mercaptoethanol), and boiled for 5 min.
Murine thymoma EL4 cells and murine CD5+ B cells, M12, were maintained in RPMI 1640 medium supplemented with 10% (v/v) 1:1 fetal calf serum and horse serum, 2 mM glutamine, 100 U/ml penicillin, 0.05 mM streptomycin, and 50 nM 2-mercaptoethanol.
The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.
1 mM 2-mercaptoethanol/1% penicillin-streptomycin) at 37°C/5% CO 2 until analysis.
5 mM Tris-HCl, 2% SDS, 10% glycerol, 10% 2-mercaptoethanol, pH 6.8) at room temperature for 30 min in preparation for SDS-PAGE.