Words similar to mercaptoethanol

Example sentences for: mercaptoethanol

How can you use “mercaptoethanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

4 mM 2-mercaptoethanol, 0.1% bromphenol blue, and 1 mM EGTA) then diluted 1:1 in PBS.
600 of 0.8 at 23°C and then diluted 1:1 with 0.4 M NaOH + 1.7% 2-mercaptoethanol.
125 M Tris, 2% SDS, 10% sucrose, 5% 2-mercaptoethanol, and 0.02% bromophenol blue at a pH of 8.0) was added to each tube and the tubes were heated at 95°C for 5 minutes.
The reaction was performed in endogenous polymerase buffer (50 mM Tris-HCl pH 8.0, 100 mM NaCl, 0.1% NP-40, 10 mM MgCl 2 2.5% 2-mercaptoethanol) with 1 μg oligonucleotide, 100 ng purified radiolabeled RNA template, and 4 U RNasin at 37°C for 65 min.
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).