Words similar to mercaptoethanol

Example sentences for: mercaptoethanol

How can you use “mercaptoethanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.
4 mM 2-mercaptoethanol, 0.1% bromphenol blue, and 1 mM EGTA) then diluted 1:1 in PBS.
These cells were cultured in RPMI medium supplemented with 10% FCS, 2 mM L-glutamine, and 0.05 mM 2-mercaptoethanol without antibiotic/antimycotic.
5 mM Tris-HCl, 2% SDS, 10% glycerol, 10% 2-mercaptoethanol, pH 6.8) at room temperature for 30 min in preparation for SDS-PAGE.
32P-labeled substrates were prepared and assays performed as described previously [ 18 ] . Briefly, protein phosphatase reactions contained 2 or 4 nM enzyme in a final volume of 30 μl containing buffer A (50 mM Tris, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% 2-mercaptoethanol), 0.4% ethanol and 10 μM radiolabeled phosphosubstrate in the absence or presence of AA [ 19 ] . Reactions were initiated by adding substrate and lipid, and incubated at 30°C for 10 to 15 min.