Words similar to mercaptoethanol

Example sentences for: mercaptoethanol

How can you use “mercaptoethanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

1 mM 2-mercaptoethanol/1% penicillin-streptomycin) at 37°C/5% CO 2 until analysis.
The eluate from the SP column was then diluted 5-fold with buffer C (20 mM tris pH 8.5, 1 mM EDTA and 0.1% 2-mercaptoethanol) and subjected to anion-exchange chromatography on a BioScale Q column (2 ml from BioRad).
Jurkat cells expressing SV40 large T antigen (Jurkat Tag) were a generous gift from Dr. Gerald Crabtree (Stanford) and were maintained in RPMI 1640 supplemented with 10% fetal calf serum, penicillin, streptomycin, glutamine, and 50 μM 2-mercaptoethanol.
Purified human AR protein over-expressed in the baculovirus [ 23 ] or nuclear protein extracts of LNCaP cells were incubated with 2 μg polydeoxynosinic-deoxycytidylic acid and 1× binding buffer (10 mM HEPES, pH 7.5, 1 mM mercaptoethanol, 10% glycerol and 50 mM KCl) on ice for 30-60 min.
The serine/threonine protein phosphatase type 5 (PP5) was purified from bovine brain according to the following procedure: a bovine brain was homogenized in ~ 3 volumes of buffer A (50 mM HEPES pH 7.5 4°C, 1 mM EDTA, 10 mM PMSF and 0.1% 2-mercaptoethanol) and subjected to centrifugation at 15,000 g for 40 min. followed by a 45%-60% ammonium sulfate fractionation of the supernatant.