Words similar to mercaptoethanol

Example sentences for: mercaptoethanol

How can you use “mercaptoethanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.
The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.
1 mM 2-mercaptoethanol/1% penicillin-streptomycin) at 37°C/5% CO 2 until analysis.
5 mM Tris-HCl, 2% SDS, 10% glycerol, 10% 2-mercaptoethanol, pH 6.8) at room temperature for 30 min in preparation for SDS-PAGE.
The serine/threonine protein phosphatase type 5 (PP5) was purified from bovine brain according to the following procedure: a bovine brain was homogenized in ~ 3 volumes of buffer A (50 mM HEPES pH 7.5 4°C, 1 mM EDTA, 10 mM PMSF and 0.1% 2-mercaptoethanol) and subjected to centrifugation at 15,000 g for 40 min. followed by a 45%-60% ammonium sulfate fractionation of the supernatant.