Example sentences for: mem

How can you use “mem” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cultures were maintained in Minimum Essential Medium (MEM, GibcoBRL, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (Sigma, St. Louis, MO), 1.4 mM glutamine (GibcoBRL, Grand Island, NY) and 20 μM 2-mercaptoethanol (Sigma, St. Louis, MO).

  • Twenty-four hours prior to fixation, the cells were incubated with 10 μM 5-bromo-2'-deoxyuridine in MEM (see Table 1).

  • In subsequent steps, motifs identified by sequence alignment programs like MEME (Multiple EM for Motif Elicitation), http://meme.sdsc.edu/meme/website/intro.htmlcan also be used to search the available databases using MAST (Motif Alignment and Search Tool, http://meme.sdsc.edu/mem/website/mast-intro.html).

  • The oligonucleotides were diluted in 100 μl Opti-MEM to various concentrations, mixed with 100 μl of Opti-MEM containing 6 μl of Lipofectamine and incubated at room temperature for 20 minutes.

  • MEM was removed and cells were detached from the substrate using 10 ml Puck's saline solution and incubated at 37°C for 5 minutes.


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