Example sentences for: mem

How can you use “mem” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Confluent hESMPCs were maintained for 2–3 wk in alpha MEM medium with 20% heat-inactivated FBS.

  • Cells grown in 96-well plates (20,000 cells/well, ~90% confluency) were treated with testing agents for a period of time as indicated in the figure legends; then, the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazole-2yl)-2,5-diphenyltetrazolium bromide) assay [ 66 ] . Culture medium was removed after treatment, then 100 μl MTT solution (100 μg/ml prepared in MEM) was added to each well.

  • Mesenchymal differentiation of hESCs (lines H1 [WA-01] and H9 [WA-09]) [9] was induced by plating undifferentiated hESCs on a monolayer of murine OP9 stromal cells [10], in the presence of 20% heat-inactivated FBS in alpha MEM medium.

  • MEM was removed and cells were detached from the substrate using 10 ml Puck's saline solution and incubated at 37°C for 5 minutes.

  • The cells were resuspended in MEM containing D-valine and 15% dialyzed fetal calf serum and plated on concavalin A-coated round glass coverslips.


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