Words similar to mem
Example sentences for: mem
How can you use “mem” in a sentence? Here are some example sentences to help you improve your vocabulary:
Differentiation of hESMPCs was induced in pellet culture [5] by exposure to 10 ng/ml TGF-β3 (R & D Systems, Minneapolis, Minnesota, United States) and 200 μM ascorbic acid (Sigma) in alpha MEM medium containing 10% FBS for 3–4 wk.
Mesenchymal differentiation was induced by plating 10 × 10 3 to 25 × 10 3 cells/cm 2 on a monolayer of OP9 cells in the presence of 20% heat-inactivated FBS in alpha MEM medium.
The drugs were then removed and incubated in calcium-containing MEM medium for 24 h prior to viability assessment using the MTT assay.
Cells grown in 96-well plates (20,000 cells/well, ~90% confluency) were treated with testing agents for a period of time as indicated in the figure legends; then, the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazole-2yl)-2,5-diphenyltetrazolium bromide) assay [ 66 ] . Culture medium was removed after treatment, then 100 μl MTT solution (100 μg/ml prepared in MEM) was added to each well.
Plastic coverslips on which the cells were cultured were sterilized by washing in ethanol, followed by rinsing three times in MEM and 15 min exposure to UV radiation.
Loading...