Words similar to mem
Example sentences for: mem
How can you use “mem” in a sentence? Here are some example sentences to help you improve your vocabulary:
Confluent hESMPCs were maintained for 2–3 wk in alpha MEM medium with 20% heat-inactivated FBS.
Cells grown in 96-well plates (20,000 cells/well, ~90% confluency) were treated with testing agents for a period of time as indicated in the figure legends; then, the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazole-2yl)-2,5-diphenyltetrazolium bromide) assay [ 66 ] . Culture medium was removed after treatment, then 100 μl MTT solution (100 μg/ml prepared in MEM) was added to each well.
Mesenchymal differentiation of hESCs (lines H1 [WA-01] and H9 [WA-09]) [9] was induced by plating undifferentiated hESCs on a monolayer of murine OP9 stromal cells [10], in the presence of 20% heat-inactivated FBS in alpha MEM medium.
MEM was removed and cells were detached from the substrate using 10 ml Puck's saline solution and incubated at 37°C for 5 minutes.
The cells were resuspended in MEM containing D-valine and 15% dialyzed fetal calf serum and plated on concavalin A-coated round glass coverslips.