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Example sentences for: meloidogyne
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Root-knot nematode species, including Meloidogyne incognita , are the most important of the plant parasitic nematodes, infecting almost all cultivated plants, and are responsible for billions of dollars in crop losses annually [ 1 2 ] . They are obligatory sedentary endoparasites with a 1- to 2-month life cycle.
Sequences were obtained from the Parasitic Nematode Sequencing Project (PNSP) [ 30 ] including clustered Meloidogyne ESTs built with the NemaGene approach [ 28 ] . We analyzed 1,799 M. incognita (WMi) sequences and 3,119 M. javanica (WMj) sequences from these PNSP clusters.
Significantly, the solitary significant match of the Meloidogyne NodL sequences to a eukaryote is to a yeast serine-acetyltransferase, an enzyme clearly separated from the RKN by function as well as in our phylogeny (Figure 2).
Alternatively, the transfer event may be more recent, such as to the progenitor of the Meloidogyne lineage since its divergence from the cyst nematodes (event 'b' in Figure 1), or in a lineage leading to a single Meloidogyne species (event 'c').
By comparison, RNAi surveys of all predicted genes on a C. elegans chromosome have found a smaller percentage of genes with phenotypes: 14% for chromosome I [ 63 ] and 13% for chromosome III [ 64 ] . Surveys of expressed genes reveal an intermediate level of 27% with phenotypes [ 65 ] . Further, selecting for C. elegans genes with expressed Meloidogyne homologs led to enrichment for genes with severe phenotypes by RNAi such as embryonic lethality or sterility as compared to the overall dataset (Figure 5) (For a complete tally of all observed phenotypes see Additional data files).
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