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The DNA sequences described in this report can be found in GenBank with the following accession numbers: Bru-VNTR-1 (clone 1) = AY099417; Bru-VNTR-2 (clone 3) = AY099419; Bru-VNTR-3 (clone 4) = AY099420; Bru-VNTR-4 (clone 5) = AY099421; Bru-VNTR-5 (clone 6) = AY099422; Bru-VNTR-6 (clone 7) = AY099423; Bru-VNTR-7 (clone 8) = AY099424; Bru-VNTR-8 (clone 9) = AY099425; Bru-VNTR-9 (clone 2) = AY099418; B. melitensis Chromosome I = NC 003317; B. melitensis Chromosome II = NC 003318; B. suis Chromosome I = NC 004310; B. suis Chromosome II = NC 004311.
In some cases, two or more products were amplified from a single locus [e.g.VNTR Locus-7 in B. melitensis biovar 1 (6 and 7 repeats) and biovar 2 (11 and 12 repeats), B. canis (10 and 11 repeats), and B. suis biovar 3 (5 and 6 repeats)].
Since the published B. melitensis and B. suis genomes are numbered in opposite orientation, the relevant strand for each genome was also listed in Table 1.
The one repeat locus that lacks this sequence contains a stable segment consisting of three repeat units in B. abortus , B. melitensis and B. suis .
Unexpectedly, different alleles were found at some loci than were predicted from the published genome sequences of B. melitensis and B. suis . This was despite using the same strains for our studies.