Example sentences for: medium

How can you use “medium” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For determination of basal gene-expression profiles without androgen stimulation, fibroblasts were cultured on 150-mm plastic dishes at 37°C with 5% CO 2 . To eliminate possible artifacts due to differing states of proliferation, cells were grown to confluence, at which point they enter G 0 arrest [ 31 ] . They were maintained in phenol-red-free DMEM F12 (Dulbecco's modified Eagle Medium with the nutrient mix F12; Gibco) containing L-glutamine, 15 mM Hepes buffer, penicillin/streptomycin (Gibco) and 12.

  • BrdU-labeled DNA fragments are released from the cells into the cytoplasm during apoptosis or into the cell culture medium during cell-mediated cytotoxicity.

  • To examine the effects of increasing doses of CDDO in human primary chondrocytes, cells were preincubated for 24 hours with CDDO (300 nM to 5 μM), or with medium alone, before the addition of IL-1 for 18 hours.

  • RPMI 1640 culture medium was supplemented with 5% heat inactivated FCS, 2 mM glutamine, 5 mM HEPES, 5 × 10 -5M 2-ME, 100 U/ml penicillin, 20 μg/ml gentamycin (pH 7.2-7.

  • The blastocysts were placed on slides with a drop of mounting medium consisting of glycerol and PBS (9:1) containing 0.1 mg/ml Hoechst 33342.


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