Words similar to mcl-
Example sentences for: mcl-
How can you use “mcl-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Endotoxin administration induces Mcl-1 expression in adherent vascular lung neutrophils
NF-κB, CREB and C/EBP are transcription factors known to be important in regulating the expression of various genes controlling apoptosis and inflammatory mediator production including Mcl-1, COX-2 and TNF-α [ 20 21 ] , and these were next examined.
This is consistent with the relatively high levels of apoptosis observed in these cells and their reduced survival in culture [ 29 ] . Our findings are in accord with a recent study demonstrating that Mcl-1 expression in human peripheral blood neutrophils decreases with aging [ 15 ] . As previously reported for human peripheral blood neutrophils [ 30 ] , we were unable to detect Bcl-2 in rat adherent vascular lung neutrophils, even after endotoxin treatment of animals.
The proteins were transferred to nitrocellulose paper, and incubated at room temperature for 3 hours, or overnight, at 4°C, with mouse monoclonal anti-human Mcl-1 (1:1000), p38 MAP kinase, phosphoinositide 3 kinase (PI3K), or protein kinase B-alpha (PKB-α) (1:500) antibodies (Transduction Laboratories, San Diego, CA, USA and New England Biolabs, Inc., Beverly, MA, USA), or rabbit polyclonal anti-rat p44/42 MAP kinase (1:1000), or doubly phosphorylated anti-human Thr180/Tyr182 phospho-p38 MAP kinase (1:1000) antibodies (New England Biolabs, Inc.).
Although expression of Mcl-1 in the neutrophils declined with time, significant amounts were still detectable 48 hours after endotoxin administration.
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