Words similar to mbt-

Example sentences for: mbt-

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In order to understand the induction kinetics of the inducible system, cell clone MBT-2+RHeoSwitch-2+luc-5B3 was treated with GS-E and cells were harvested at different times to determine the time course of gene induction.
The sensitivity of the gene expression system to varying doses of GS-E was determined by treating duplicate samples of clone MBT-2+RHeoSwitch-2+luc-5B3 with varying concentrations of GS-E for 24 or 48 hours and dual luciferase assays were performed on the cell lysates (Figure 3B).
Stably transfected MBT-2 cells were selected in media containing 500 μg/ml G418 and Panc02 cells in 700 μg/ml G418 for three weeks after which there were many large cell colonies on each transfection plate.
Following transfection, cells were allowed to recover for 24 h prior and were then selected in 500 μg/ml G418 for MBT-2 cells and 700 μg/ml G418 for Panc02 cells for three weeks.
After treatment with 15 μM GS-E, three of the four MBT-2 clonal cell lines had a two-fold or lower increase in luciferase activity.