Example sentences for: mbo

How can you use “mbo” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).

  • castaneus (CAST) created an Mbo II restriction site in the CAST sequence, we used RFLP analysis to assess allelic representation in cDNAs from F1 progeny of BL/6 × CAST and CAST × BL/6 reciprocal crosses.

  • The radiolabeled PCR products were digested with Alu I or Hpa II, denatured and electrophoresed for 10-16 h at 500 V on non-denaturing 6% acrylamide gels at room temperature [ 24 ] . For RFLP analysis, PCR products were digested with Mbo II and analyzed by electrophoresis on non-denaturing 6% acrylamide gels at 500 V for 3-4 hours.


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