Words similar to mannheim
Example sentences for: mannheim
How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:
cDNA was synthesized from each sample by incubating 1 ug RNA sample with 0.5 mM each dNTP, 0.25 μg/ml random hexamer (Boehringer Mannheim), 10 U RNase inhibitor, and 10 U OmniScript reverse transcriptase in OmniScript RT Buffer (Qiagen).
Subtilisin was from Boehringer Mannheim.
Membranes were washed in TBST, incubated with the appropriate secondary antibody at 1/10000 dilution (Boehringer Mannheim) for 1 hr and protein was visualized by enhanced chemiluminescence (Amersham).
Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.45 mg ml -1trypsin (Worthington, Freehold, NJ), 0.6 mg ml -1collagenase D (Boehringer Mannheim, Indianapolis, IN) and 0.05 mg ml -1DNAse I (Sigma, St. Louis, MO) for 1 hour at 35°C.
Following washing three times, cells were incubated in a (1 : 50) dilution of mouse anti-BrdU monoclonal antibody (Boehringer Mannheim, Indianapolis, IN, USA) for 2 h at 37°C.
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