Example sentences for: mannheim

How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • cDNA was synthesized from each sample by incubating 1 ug RNA sample with 0.5 mM each dNTP, 0.25 μg/ml random hexamer (Boehringer Mannheim), 10 U RNase inhibitor, and 10 U OmniScript reverse transcriptase in OmniScript RT Buffer (Qiagen).

  • Subtilisin was from Boehringer Mannheim.

  • Membranes were washed in TBST, incubated with the appropriate secondary antibody at 1/10000 dilution (Boehringer Mannheim) for 1 hr and protein was visualized by enhanced chemiluminescence (Amersham).

  • Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.45 mg ml -1trypsin (Worthington, Freehold, NJ), 0.6 mg ml -1collagenase D (Boehringer Mannheim, Indianapolis, IN) and 0.05 mg ml -1DNAse I (Sigma, St. Louis, MO) for 1 hour at 35°C.

  • Following washing three times, cells were incubated in a (1 : 50) dilution of mouse anti-BrdU monoclonal antibody (Boehringer Mannheim, Indianapolis, IN, USA) for 2 h at 37°C.


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