Example sentences for: mannheim

How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 01 μM - 1 mM final concentration) or vehicle (LPS-free water) and 10 μl of the tetrazolium salt WST-obtained from Roche Diagnostics Gmbh (Mannheim, Germany).

  • The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.

  • 1 M maleic acid, 3 M NaCl, 0.3% Tween 20, pH 8) at room temperature followed by blocking for two hours at room temperature in maleate buffer with 0.5% blocking reagent (Boehringer Mannheim, Biochemical Products, Indianapolis IN).

  • Membranes were initially prepared and B max and K d values determined in the presence and absence of a cocktail of protease inhibitors (Complete Protease Inhibitor Cocktail, Roche Diagnostics, Mannheim Germany).

  • After incubation in the presence of cell-death-inducing reagents, Jurkat cells were centrifuged at 200 × g for 30 min, followed by one wash at 1000 × g for 10 min in PBS containing Complete Protease Inhibitor cocktail (Roche, Mannheim, Germany).


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