Example sentences for: mannheim

How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The diagnosis of each patient is shown in Table 1. Tissue samples were homogenized in a Dounce homogenizer on ice in 1 ml of lysis buffer (25 mM MES, 1 mM MgSO 4 , 2 mM EGTA, 0.1 mM GTP, 0.1% Triton X-100 with a protease inhibitor cocktail [Complete Protease Inhibitor Tablets; Boehringer Mannheim]).

  • The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).

  • Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.45 mg ml -1trypsin (Worthington, Freehold, NJ), 0.6 mg ml -1collagenase D (Boehringer Mannheim, Indianapolis, IN) and 0.05 mg ml -1DNAse I (Sigma, St. Louis, MO) for 1 hour at 35°C.

  • Antibodies against the following proteins were obtained from the following suppliers or individuals: ant-phosphotyrosine, RC20-HRP (Transduction Laboratories, Lexington, KY); anti-HA (Boehringer Mannheim, Indianapolis, IN); anti-gag (Dr.

  • Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).


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