Words similar to mannheim
Example sentences for: mannheim
How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).
1 M maleic acid, 3 M NaCl, 0.3% Tween 20, pH 8) at room temperature followed by blocking for two hours at room temperature in maleate buffer with 0.5% blocking reagent (Boehringer Mannheim, Biochemical Products, Indianapolis IN).
Membranes were washed in TBST, incubated with the appropriate secondary antibody at 1/10000 dilution (Boehringer Mannheim) for 1 hr and protein was visualized by enhanced chemiluminescence (Amersham).
Incubation was then performed for ten minutes at room temperature with CDP-Star chemiluminescent substrate (Boehringer Mannheim, Biochemical Products, Indianapolis IN) diluted 1:100 in substrate buffer.
01 μM - 1 mM final concentration) or vehicle (LPS-free water) and 10 μl of the tetrazolium salt WST-obtained from Roche Diagnostics Gmbh (Mannheim, Germany).
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