Words similar to mannheim
Example sentences for: mannheim
How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:
E. coli bacteria containing pHIP-based plasmids were grown in LB containing 200 μg hygromycin/mL (Boehringer Mannheim Biochemicals, Indianapolis, Ind.).
Low molecular weight protein markers (Boehringer Mannheim/Roche Molecular Biochemicals, Indianapolis, IN) were used as migration standards.
Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.45 mg ml -1trypsin (Worthington, Freehold, NJ), 0.6 mg ml -1collagenase D (Boehringer Mannheim, Indianapolis, IN) and 0.05 mg ml -1DNAse I (Sigma, St. Louis, MO) for 1 hour at 35°C.
Detailed procedures for isolation of total RNA, enrichment of poly (A)+ RNA by oligo-dT cellulose chromatography and electrophoresis and Northern blotting of RNA have been described previously [ 13 ] . The cDNA probes of mouse RAR α and RAR γ [ 21 22 ] were isolated by agarose electrophoresis after appropriate endonucleases digestion and were radio-labeled with 32P-dCTP, using random priming kit (Boehringer-Mannheim, Indianapolis, IN).
Incubation was then performed for ten minutes at room temperature with CDP-Star chemiluminescent substrate (Boehringer Mannheim, Biochemical Products, Indianapolis IN) diluted 1:100 in substrate buffer.
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