Words similar to mannheim
Example sentences for: mannheim
How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:
, 0.1% SDS, and 1.0% Tween 20 for 45 min, washed, and reprobed with antibodies raised against other proteins of interest, e.g., the first membrane with ERα antibody ID5 (DAKO) and the second membrane with actin mouse monoclonal antibody, clone C4 (Boehringer Mannheim Corp., Indianapolis, IN) 0.25 μg/ml.
Membranes were initially prepared and B max and K d values determined in the presence and absence of a cocktail of protease inhibitors (Complete Protease Inhibitor Cocktail, Roche Diagnostics, Mannheim Germany).
Further, tissues were cut into smaller pieces and incubated at 37°C in Isocove's medium supplemented with 10% fetal calf serum and penicillin/streptomycin containing 400 U/ml Collagenase D (Boerrhinghem & Mannheim GmbH, Mannheim, Germany) and 25 U/ml DNAse (Worthington Biochemical, Lakewood, NJ) for 2-3 hours.
25 μg/ml in blocking reagent, clone C4, Boehringer Mannheim Corp., Indianapolis, IN).
Subtilisin was from Boehringer Mannheim.
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