Words similar to mannheim
Example sentences for: mannheim
How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:
The diagnosis of each patient is shown in Table 1. Tissue samples were homogenized in a Dounce homogenizer on ice in 1 ml of lysis buffer (25 mM MES, 1 mM MgSO 4 , 2 mM EGTA, 0.1 mM GTP, 0.1% Triton X-100 with a protease inhibitor cocktail [Complete Protease Inhibitor Tablets; Boehringer Mannheim]).
The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).
Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.45 mg ml -1trypsin (Worthington, Freehold, NJ), 0.6 mg ml -1collagenase D (Boehringer Mannheim, Indianapolis, IN) and 0.05 mg ml -1DNAse I (Sigma, St. Louis, MO) for 1 hour at 35°C.
Antibodies against the following proteins were obtained from the following suppliers or individuals: ant-phosphotyrosine, RC20-HRP (Transduction Laboratories, Lexington, KY); anti-HA (Boehringer Mannheim, Indianapolis, IN); anti-gag (Dr.
Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).