Example sentences for: mannheim

How can you use “mannheim” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).

  • The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).

  • , 0.1% SDS, and 1.0% Tween 20 for 45 min, washed, and reprobed with antibodies raised against other proteins of interest, e.g., the first membrane with ERα antibody ID5 (DAKO) and the second membrane with actin mouse monoclonal antibody, clone C4 (Boehringer Mannheim Corp., Indianapolis, IN) 0.25 μg/ml.

  • 1 M maleic acid, 3 M NaCl, 0.3% Tween 20, pH 8) at room temperature followed by blocking for two hours at room temperature in maleate buffer with 0.5% blocking reagent (Boehringer Mannheim, Biochemical Products, Indianapolis IN).

  • All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).


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