Words similar to magnesium
Example sentences for: magnesium
How can you use “magnesium” in a sentence? Here are some example sentences to help you improve your vocabulary:
1, 100 mM potassium acetate, 30 mM magnesium acetate), and brought up in hybridization mix according to the manufacturer's protocols (1 M NaCl, 10 mM Tris-acetate pH8.
There are two homologous type IA topoisomerases present in E. coli . Topoisomerase III has potent DNA decatenating activity for resolution of plasmid DNA replication intermediates, but much weaker relaxation activity than topoisomerase I [ 17 ] . To exhibit maximal relaxation activity, topoisomerase III requires high temperature (52°C) along with low magnesium and monovalent ion [ 17 18 ] . In contrast, E. coli topoisomerase I was not active in the in vitro assay for resolution of plasmid DNA replication intermediates [ 19 ] . Removal of the C-terminal 49 amino acids from the 653 amino acid topoisomerase III protein resulted in drastic reduction of catalytic activity [ 20 ] . Fusion of the carboxyl-terminal 312 amino acid residues of E. coli topoisomerase I, which includes the entire ZD domain, onto the 605 N-terminal amino acids of topoisomerase III generated a hybrid topoisomerase that has relaxation activity resembling topoisomerase III along with weak decatenating activity [ 21 ] . Although preferring single-stranded DNA as binding substrate, topoisomerase I had been shown to also bind double-stranded DNA [ 22 ] , but there is no data available to indicate which domain in the enzyme is responsible for this interaction.
Magnesium had previously been shown to suppress structural defects in the td intron, and ligand-induced suppressions of splicing defects were examined by carrying out splicing reactions at either low (3 mM, stringent) or high (8 mM, permissive) magnesium concentrations [ 15 ] . Several of the constructs were either relatively inactive (e.g.
For all of the aptazymes except Th3P6, the ligand-induced splicing activity was greater in a standard assay at the more stringent (3 mM) magnesium concentration (Table 1).
The cells were lysed with HMK-NP40 (50 mM Hepes, pH 7.5, 40 mM magnesium acetate, 20 mM potassium phosphate, 5% sucrose, 0.4% NP-40), clarified, and protein concentration determined as above.
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