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Example sentences for: macvector
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7 (Molecular Biology Insights, Inc., Cascade, CO) and MacVector v7.
Primers used were based upon previously published mouse primer sets [ 27 28 ] , or designed using MacVector from cDNA sequences between exons, avoiding the amplification of potentially contaminating genomic DNA in the total RNA sample (Table 3).
Each gene and each predicted gene product was also subjected to gap BLASTX and BLASTN respectively http://www.ncbi.nlm.nih.gov/BLAST/ [ 20 ] . The similarity between two different DNA sequences was determined using the BLAST 2 sequence program from http://www.ncbi.nlm.nih.gov/blast/bl2seq/bl2.html [ 21 ] . Hydrophilicity profiles of the gene product of each Cpn 1054 family member was determined using hydropathy plot analysis ( [ 22 ] MacVector™).
The data was analyzed using the DNAStar (Madison, WI) package and Artemis [ 15 ] . Rates of synonymous and non-synonymous substitution were calculated by the un-weighted method of Nei and Gojobori [ 27 ] . Pustell DNA matrix analysis [ 25 ] was performed using MacVector version 7.1 software.
MacVector software was used for sequence analysis in this study.