Example sentences for: lyophilization

How can you use “lyophilization” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To eliminate the lyophilization step from the protocol, we used an alternative column-the Sephadex™ G-50 column-to filter out free nucleotides from the ds cDNA after completion of the second strand synthesis reaction.

  • Cy3- and Cy5-labeled cDNA targets were then purified on QIAquick PCR purification columns, combined and concentrated by lyophilization, and hybridized to the microarray at 42°C for 16 h in hybridization solution containing 50% formamide, 5x SSC, 0.1% SDS, 20 μg mouse Cot-1 DNA and 10 μg poly(dA).

  • A drawback to this method is that the cDNA is eluted with a large volume and needs to be concentrated into a much smaller volume by lyophilization prior to in vitro transcription.


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