Words similar to lps-free

Example sentences for: lps-free

How can you use “lps-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

For the O 2 - assay, rat neonatal microglia (250,000 cells/ 24-well cell culture clusters) were treated with (1) 1 mM domoic acid, (2) LPS-free water used as a vehicle for domoic acid or (3) 10 ng/ml LPS in a final volume of 1 ml of DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S and incubated in a humidified 5% CO 2 incubator at 36°C for 1 to 6 hours.
Briefly, rat neonatal microglia or cerebellar granule cells (60,000 cells / well) were plated in LPS-free 96-well cell culture clusters 24 hours prior to the experiments in either 0.1 ml DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S (microglia), or 0.1 ml serum-free Neurobasal A medium with B27 supplement (cerebellar granule neurons).
LPS B (Escherichia coli 026:B6) was obtained from Difco Laboratories (Detroit, MI); crystalline lyophilized domoic acid was purchased from Diagnostics Chemicals Ltd. (Oxford, CT) and was dissolved in LPS-free water obtained from GIBCO-BRL (Grand Island, NY) to prepare a 12.
01 μM - 1 mM final concentration) or vehicle (LPS-free water) and 10 μl of the tetrazolium salt WST-obtained from Roche Diagnostics Gmbh (Mannheim, Germany).
5 mM) and dilutions prepared with LPS-free water was confirmed by electrospray mass spectrometry prior to the experiments.