Words similar to loxp

Example sentences for: loxp

How can you use “loxp” in a sentence? Here are some example sentences to help you improve your vocabulary:

Importantly, the pgk promoter of this cassette is flanked by loxP elements so that the promoter can easily be removed using Cre recombinase.
More recently, transgenic mice have also been used to express Cre recombinase to mediate the conditional deletion of loxP -flanked DNA sequences in specific cell types in vivo . Traditional methods for generating transgenic mice require the availability of well characterized transcriptional regulatory elements, promoters or enhancers, that direct transgene expression in specific cell types.
4 kb cassette containing the 5' end of Neo coding sequence accompanied by the pgk promoter with flanking loxP elements was introduced into the SphI site (blunt) of pNEB193 (New England Biolabs).
The loxP - neo -tpA- loxP was excised from plasmid PGKneotpAlox2 by cutting with NotI, Klenow filling, and then cutting with KpnI.
A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.