Words similar to low-glucose
Example sentences for: low-glucose
How can you use “low-glucose” in a sentence? Here are some example sentences to help you improve your vocabulary:
Examples include genes for the yeast hexose transporter, whose amplification has been shown to increase fitness in a low-glucose environment [ 49], the nematode P-glycoprotein gene, the classical eukaryotic multidrug-resistance gene [ 36], and the fruit fly copper-binding metallothionein gene whose duplication is implicated in copper tolerance [ 40, 41].
As shown below, insulin expression in NS-derived cells required culture in low-glucose medium at stage 2, but we did not consistently detect insulin + cells by immunohistochemistry until stage 4.
Culture medium was prepared with low-glucose Dulbecco's modified Eagle medium with 10mmol/l HEPES (GIBCO BRL, Grand Island, NY, USA), 0.1mmol/l nonessential amino acids (Sigma Chemical, St. Louis, MO, USA), 0.4 mmol/l L-proline (Sigma Chemical), and 1mmol/l gadolinium-DTPA (Magnevist, Berlex Imaging, Wayne, NJ, USA).
Pdx1, Cdx1, and FoxA3 expression was induced in “stage 3,” when cell clusters were allowed to adhere to pre-coated wells at a density of 40–80 NSs/cm 2 and cultured in low-glucose medium with 2 μM RA for 2 wk (see Figure 1A and 1C).
These were cultured for 2 wk (stage 3) in a 1:1 mixture of low-glucose DMEM and F-12 media containing 100 mg/l apo-transferrin (Sigma), 288 mg/l glucose, 73 mg/l L -glutamine, 1.69 g/l sodium bicarbonate, 25 μg/ml bovine insulin (Sigma), 20 nM progesterone, 100 μM putrescine, 30 nM sodium selenite, and penicillin/streptomycin.