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Example sentences for: lncap
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Sub-confluent (80-90%) LNCaP cells propagated in T25 flasks were washed with 5 ml of plain RPMI 1640 medium and trypsinized with 5 ml trypsin/EDTA solution (GIBCO-BRL, Gaithersburg, MD).
Unlike in rat fibroblasts and human U87 cells [ 29 30 32 ] , SKP2 overexpression is not sufficient to override androgen-induced G1 arrest in LNCaP cells, despite a profound effect on p27 levels (Fig.
We were curious to know whether the expression changes observed in LNCaP would also occur in other androgen-responsive human prostate cancer cell lines (MDA PCa 2a, MDA PCa 2b, and LAPC-4), or in LNCaP and LAPC-4 subclones carried in androgen-depleted conditions for extended periods.
These results suggest that RAR and EGF-R signaling events might be involved in the effects of RA and T on LNCaP cell growth.
Responses to androgen of the set of transcripts identified as androgen-responsive in the LNCaP experiments were examined in each of these cell lines.