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Example sentences for: lncap
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Both substrates were incubated with recombinant cyclin E/CDK2 complexes in the presence of ATP, ubiquitin, and LNCaP cell lysate.
Treatment with 1 nM R1881 promotes proliferation in the MDA PCa 2a and 2b cell lines, has no proliferative effect in the LNCaP and LAPC-4 cell lines, and represses proliferation in LNCaP cell lines carried for prolonged periods in conditions of androgen deprivation (data not shown, and see references in [ 17, 18, 19]).
Incubation of nuclear extracts of LNCaP cells with the 32P-oligo probe also resulted in a complex that migrated to the same distance as that achieved by AR- 32P-oligo probe complex (Figure 4B).
LNCaP-FGC cells were obtained from ATCC (order number CRL-10995) and maintained in RPMI supplemented with 10% FBS as described [ 46 ] . We noticed that LNCaP cells at low density grow best on 15 cm Falcon 3025 tissue culture dishes and that the effects of SKP2 overexpression were most pronounced when cells were cultured on these dishes (data not shown).
In our initial experiments, we focused exclusively on the well-characterized LNCaP human prostate cancer cell line.