Words similar to lithium
Example sentences for: lithium
How can you use “lithium” in a sentence? Here are some example sentences to help you improve your vocabulary:
This DNA was linearized with Kpn I and Sac I and then transformed into the diploid C. albicans strain BWP17 using the lithium acetate method.
Yeast media and procedures were as described [ 43 44 ] . S. cerevisiae transformations were carried out by the lithium acetate method [ 45 ] . E. coli strain XL-1 Blue was used for bacterial cloning and plasmid amplification.
8% H 2 O, 1% lithium citrate, 1% thiodiglycol, 0.7% hydrogen chloride, 0.5% benzoic acid, pH 2.2), transferred to an Amicon 30 microconcentrator (Amicon Inc., Beverly, MA), and centrifuged at 16060 × g for 60 minutes at 4°C.
The transformations were performed using the lithium acetate method [ 18 ] . The integrants were selected at 30°C on YE plates containing 200 μg/ml G418.
Expression of the capping enzymes from these plasmids is driven by the nmt1* promoter [ 19 ] . The plasmids were transformed into heterozygous pct1 +/ pct1::kanMX or pce1 +/ pct1::kanMX diploids using the lithium acetate method [ 18 ] . The Leu +diploid transformants were then sporulated on ME plates at room temperature.